國家衛生研究院 NHRI:Item 3990099045/4670
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    题名: Development of an online-cleanup liquid chromatography tandem mass spectrometry method for the analysis of N-acetyl-S-(propionamide) cysteine in human urine
    作者: Li, CM;Wu, KY
    贡献者: Division of Environmental Health and Occupational Medicine
    摘要: Acrylamide was reported present in high-temperature fried foods. Daily intake of foods may lead to various significant exposures. In vivo uptake of acrylamide may be subjected to conjugation with glutathione under the catalysis of glutathione transferase. This conjugated product can be excreted through urine and considered as a biomarker for acrylamide exposure. The objective of this study was to develop a highly specific, rapid, and sensitive method to quantitatively analyze urinary N-acetyl-S-(propionamide) cysteine (NASPC) to serve as a biomarker for the assessment of acryl-amide exposures. After mineral salt was precipitated with acetonitrile, urine samples were cleaned up using an automatic column-switching device coupled with a betasil diol cartridge as a solid-phase extraction column. The fraction containing NASPC was back-flushed on to a microbore betasil diol column for final chromatography and detected by a mass spectrometer, that consisted of an electrospray ion source and a triple-stage-quadrupole mass analyzer using multiple reaction monitoring (MRM) mode. The detection limit of this method was estimated to be lower than 0.02 ug/mL. The calibration curve was linear using human urine spiked at 0.05 ug/mL to 5.0 ug/mL. These results demonstrated the potential application for high-throughput and accurate determina-tion of N-acetyl-S-(propionamide) cysteine levels to assess exposure for future study on the acrylamide associated adverse health effects.
    日期: 2004-08
    關聯: Drug Metabolism Reviews. 2004 Aug;36(Suppl. 1):41.
    Link to: http://dx.doi.org/10.1081/DMR-200027024
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0360-2532&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000224023200082
    显示于类别:[吳焜裕(2001-2008)] 會議論文/會議摘要

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