國家衛生研究院 NHRI:Item 3990099045/4717

NHRI > Institute of Molecular and Genomic Medicine > Chi-Ying F. Huang(1998-2005) > Conference Papers/Meeting Abstract > Item 3990099045/4717

Please use this identifier to cite or link to this item: http://ir.nhri.org.tw/handle/3990099045/4717

Title:	The interplay of hNinein and Astrin- hNinein is required for targeting Spindle-associated protein Astrin to the centrosome
Authors:	Cheng, T;Hsiao, Y;Lin, C;Hsu, C;Chang, M;Lee, C;Yu, RC;Huang, CF;Howng, S;Hong, Y
Contributors:	Division of Molecular and Genomic Medicine
Abstract:	Human Ninein (hNinein) is implicated in centrosomal microtubulenucleation and microtubule anchoring in interphase cells and may actas scaffold protein, but its interaction partner(s) is still poorlyunderstood. In this report, we show clearly that a spindle-associatedprotein Astrin could interact and co-localize with hNinein at thecentrosome during S and G2 phase of the cell cycle. In addition,hNinein truncated forms are able to affect Astrin localization to the centrosome, supporting the idea that Astrin is possibly localized at the centrosome via interaction with hNinein. Moreover, siRNA-mediatedknockdown of hNinein in HeLa cells causes Astrin to fail to localize atthe centrosome and block mitotic entry, whereas depletion of Astrin bysiRNA shows no detectable influence on the localization of hNinein to the centrosome. Remarkably, reduction in Astrin by siRNA-mediatedknockdown might result from uneven centrosome fragmentation andaneuploidy. Taken together, we propose a model where hNinein isrequired for Astrin targeting to the centrosome for the regulation of the dynamic movement of Astrin throughout the cell cycle and for maintaining centrosome/spindle pole integrity.
Date:	2006
Relation:	Molecular Biology of the Cell. 2006;17(Suppl. S):Abstract no L41.
Link to:	http://ascb.org/files/Past-AM-Meetings/2006_Late_Abstracts.pdf
JIF/Ranking 2023:	http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=1059-1524&DestApp=IC2JCR
Cited Times(WOS):	https://www.webofscience.com/wos/woscc/full-record/WOS:000207130700042
Appears in Collections:	[Chi-Ying F. Huang(1998-2005)] Conference Papers/Meeting Abstract

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