國家衛生研究院 NHRI:Item 3990099045/4792

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Title:	The protease-mediated nucleus shuttles of subnanometer gold quantum dots for real-time monitoring of apoptotic cell death
Authors:	Lin, SY;Chen, NT;Sun, SP;Chang, JC;Wang, YC;Yang, CS;Lo, LW
Contributors:	Center for Nanomedicine Research;Division of Medical Engineering Research
Abstract:	Subnanometer photoluminescent gold quantum dots (GQDs) are functionalized with a peptide moiety that contains both nuclear export signal (NES) and nuclear localization signal (NLS) sequences. By taking advantage of its small size and great photostability, the functionalized GQDs are used to mimic the actions of nucleus shuttle proteins, especially of those activated during cell apoptotic death, to work as protease-mediated cytoplasm-nucleus shuttles for dynamic monitoring of apoptosis. The resulting construct demonstrates activation of the nuclear pore complex (NPC) of cells, for bidirectional transport between nucleus and cytoplasm. A capsase-3 recognition sequence (DEVD), placed within the NLS/NES peptide, serves as a proteolytic site for activated caspase-3. Upon the induction of apoptosis, the activated caspase-3 cleaves the functional peptide on GQDs resulting in changes of subcellular distribution of GQDs. Such changes can be quantified as a function of time, by the ratios of GQDs photoluminescence in nucleus to that in cytoplasm. As such, the NES-linker-DEVD-linker-NLS peptide enables the GQDs to function as molecular probes for the real-time monitoring of cellular apoptosis.
Date:	2010-06-23
Relation:	Journal of the American Chemical Society. 2010 Jun 23;132(24):8309-8315.
Link to:	http://dx.doi.org/10.1021/ja100561k
JIF/Ranking 2023:	http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0002-7863&DestApp=IC2JCR
Cited Times(WOS):	https://www.webofscience.com/wos/woscc/full-record/WOS:000278905700029
Cited Times(Scopus):	http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=77953641803
Appears in Collections:	[Chung-Shi Yang] Periodical Articles [Shu-Yi Lin] Periodical Articles [Leu-Wei Lo] Periodical Articles

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