國家衛生研究院 NHRI:Item 3990099045/4971

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Title:	Contribution of a plasmid-borne blaOXA-58 gene with its hybrid promoter provided by IS1006 and an ISAba3-like element to beta-lactam resistance in acinetobacter genomic species 13TU
Other Titles:	Contribution of a plasmid-borne blaOXA-58 gene with its hybrid promoter provided by IS1006 and an ISAba3-Like element to β-Lactam resistance in acinetobacter genomic species 13TU
Authors:	Chen, TL;Chang, WC;Kuo, SC;Lee, YT;Chen, CP;Siu, LK;Cho, WL;Fung, CP
Contributors:	Division of Infectious Diseases
Abstract:	The contribution of the bla(OXA-58) gene and its promoter to beta-lactam resistance has not been validated in Acinetobacter spp. other than Acinetobacter baumannii. We identified a multidrug-resistant (including carbapenem resistance) Acinetobacter genomic species 13TU in which bla(OXA-58) was the only detected carbapenemase gene. The bla(OXA-58) gene was plasmid located, flanked by ISAba3 (downstream) and an ISAba3-like element (upstream). An IS1006 element was inserted into ISAba3-like (IS1006-Delta ISAba3-like) to generate a hybrid promoter for bla(OXA-58), with a -35 promoter located in IS1006 and a -10 promoter in ISAba3-like. The reference strain of Acinetobacter genomic species 13TU, ATCC 17903, revealed higher MICs of amoxicillin, ticarcillin, and piperacillin and heteroresistance to imipenem and meropenem when it was transformed with a shuttle vector containing a fragment encompassing Delta ISAba3-like-bla(OXA-58), compared to the same host containing only bla(OXA-58). When the fragment was changed from similar to ISAba3like-bla(OXA-58) to IS1006-Delta ISAba3-like-bla(OXA-58), the ATCC 17903 transformant revealed a markedly higher level of bla(OXA-58) transcription (12-fold), increased cefuroxime and piperacillin-tazobactam MICs, and homoresistance to imipenem and meropenem. Different roles of the insertion elements preceding the blaOXA-58 gene in Acinetobacter genomic species 13TU are demonstrated. The ISAba3-like-bla(OXA-58) construct can mediate resistance to penicillin derivatives but only heteroresistance to carbapenems. The insertion of IS1006 into ISAba3-like, generating a hybrid promoter, could further enhance the transcription of bla(OXA-58) and mediate homoresistance to carbapenems and also enhanced resistance to piperacillin-tazobactam.
Date:	2010-08
Relation:	Antimicrobial Agents and Chemotherapy. 2010 Aug;54(8):3107-3112.
Link to:	http://dx.doi.org/10.1128/aac.00128-10
JIF/Ranking 2023:	http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0066-4804&DestApp=IC2JCR
Cited Times(WOS):	https://www.webofscience.com/wos/woscc/full-record/WOS:000279963300005
Cited Times(Scopus):	http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=77955389745
Appears in Collections:	[Leung-Kei Siu] Periodical Articles

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