國家衛生研究院 NHRI:Item 3990099045/5041
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    题名: A recombinant peroxisome proliferator response element-driven luciferase assay for evaluation of potential environmental obesogens
    作者: Wang, YF;Chao, HR;Wu, CH;Tseng, CH;Kuo, YT;Tsou, TC
    贡献者: Division of Environmental Health and Occupational Medicine
    摘要: A recombinant Huh7-PPRE-Luc cell line for analyzing the peroxisome proliferator response element (PPRE)-driven luciferase activity was established. The cells exhibited a good dose-response induction in PPRE-driven luciferase activity by three subtypes of peroxisome proliferator-activated receptor (PPAR) agonists as well as by a retinoid X receptor agonist, 9-cis-retinoic acid. Among five environmental chemicals tested, benzyl butyl phthalate and bisphenol induced PPRE-driven luciferase activation in Huh7-PPRE-Luc cells and caused adipogenic differentiation of 3T3-L1 cells. This recombinant Huh7-PPRE-Luc cell line would be useful for screening potential environmental obesogens with PPAR activity.
    日期: 2010-12
    關聯: Biotechnology Letters. 2010 Dec;32(12):1789-1796.
    Link to: http://dx.doi.org/10.1007/s10529-010-0359-9
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0141-5492&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000285912900004
    Cited Times(Scopus): http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=78149406600
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