國家衛生研究院 NHRI:Item 3990099045/5347
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    题名: Tumor necrosis factor-alpha, Interleukin-8 and Interleukin-6 are involved in vascular endothelial cell capillary tube and network formation induced by tumor-associated macrophages
    其它题名: Tumor necrosis factor-α, Interleukin-8 and Interleukin-6 are involved in vascular endothelial cell capillary tube and network formation induced by tumor-associated macrophages
    作者: Lee, CC;Liu, KJ;Chen, LL;Wu, YC;Huang, TS
    贡献者: National Institute of Cancer Research
    摘要: AIM: The goal of this study is to investigate the involvement of inflammatory cytokines produced by tumor-associated macrophages in promoting tumor angiogenesis. METHODS: To study the angiogenic effect of tumor-associated macrophages (TAMs), we overlaid human umbilical vein endothelial cells on top of Matrigel containing MCF-7 breast cancer cells with or without macrophages and investigated the outcome of endothelial cell capillary tube and network formation. We also determined the levels of interleukin (IL)-1beta, IL-6, IL-8, IL-10, IL-12p70, tumor necrosis factor-alpha (TNF-alpha), and vascular endothelial growth factor (VEGF) in the media of MCF-7 breast cancer cells co-cultivated with or without macrophages. Furthermore, anti-IL-8 receptor antagonizing antibody, IL-6 or TNF-alpha soluble receptor, and inhibitors against NF-kB, MEK, p38MAPK, and JNK, respectively, were used to determine which signal transduction pathways are involved in TAMs-induced angiogenic activity. RESULTS: The Matrigel mixed with MCF-7 cells and macrophages was more efficient than 100 ng/ml of VEGF to induce vascular endothelial cell tube and network formation. The expression of IL-6, IL-8 and TNF-alpha were significantly enhanced by co-cultivation of MCF-7 cells with macrophages. The promotion of capillary tube and network formation by TAMs was inhibited either with anti-IL-8 receptor antagonizing antibody or with IL-6 or TNF-alpha soluble receptor, suggesting that IL-8, TNF-alpha and IL-6 indeed participated in TAMs-induced angiogenesis. In addition, TAMs-induced angiogenic activity could also be attenuated by the presence of inhibitors against NF-kB, ERK, and p38MAPK signaling pathways. CONCLUSION: IL-8, TNF-alpha and IL-6 were involved in TAMs-associated angiogenesis via NF-kB, ERK, and p38MAPK-dependent signaling pathways.
    日期: 2006-08
    關聯: Journal of Cancer Molecules. 2006 Aug;2(4):155-160.
    Link to: http://mupnet.com/JOCM%202%284%29%20155.htm
    显示于类别:[劉柯俊] 期刊論文
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