國家衛生研究院 NHRI:Item 3990099045/5835

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Title:	Tylophorine elevates the amounts of c-Jun protein in carcinoma cells
Authors:	Lee, SJ;Wu, CM;Yang, CW
Contributors:	Institute of Biotechnology and Pharmaceutical Research
Abstract:	Tylophorine has been reported to inhibit the growth of a variety of carcinoma cells in vitro and exert anti-cancer activity in vivo. The underlying mechanisms are not clear as yet. We also found tylophorine exerted potent inhibition in the growth of human HepG2, HONE-1 and NUGC-3 carcinoma cells, with IC50s of ~240, ~120, and ~160nM, respectively. Interestingly, tylophorine treatment elevated the amount of c-Jun protein in these three types of carcinoma cells while the amount of ATF2 protein, another key component of AP1, remained un-changed. AP-1, a transcription factor complex, has been correlated with cell survival and proliferation, Although both phosphorylated c-Jun and ATF2 increased upon the tylophorine treatment, the population of phosphorylated c-Jun seemed to not increase significantly. On the other hand, from the results of in vitro kinase assay and co-treatment of tylophorine with SP600125 (JNK inhibitor), SB203580 (p38 inhibitor), we found that the phosphorylation of c-Jun was mainly contributed by JNK kinase activity, but the phosphorylation of ATF-2 was associated with both p38 and JNK activity. Moreover, the effects of tylophorine on the post-translational modification of c-Jun and AP1 activity/function are being under investigation. In addition, the effect of elevated c-Jun protein amount in anti-growth of carcinoma cells will be studied.
Date:	2007-12
Relation:	ASCB 47th Annual Meeting. 2007 Dec 1-5.
Link to:	http://ascb.org/files/Past-AM-Meetings/2007_Abstracts.pdf
Appears in Collections:	[Shiow-Ju Lee] Conference Papers/Meeting Abstract

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