國家衛生研究院 NHRI:Item 3990099045/5933
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    Please use this identifier to cite or link to this item: http://ir.nhri.org.tw/handle/3990099045/5933


    Title: Targeting PML-RAR alpha and oncogenic signaling pathways by Chinese herbal mixture Tien-Hsien liquid in acute promyelocytic leukemia NB4 cells
    Other Titles: Targeting PML-RARα and oncogenic signaling pathways by Chinese herbal mixture Tien-Hsien liquid in acute promyelocytic leukemia NB4 cells
    Authors: Yao, CJ;Yang, CM;Chuang, SE;Yan, JL;Liu, CY;Chen, SW;Yan, KH;Lai, TY;Lai, GM
    Contributors: National Institute of Cancer Research
    Abstract: Tien-Hsien Liquid (THL) is a Chinese herbal mixture that has been used worldwide as complementary treatment for cancer patients in the past decade. Recently, THL has been shown to induce apoptosis in various types of solid tumor cells in vitro. However, the underlying molecular mechanisms have not yet been well elucidated. In this study, we explored the effects of THL on acute promyelocytic leukemia (APL) NB4 cells, which could be effectively treated by some traditional Chinese remedies containing arsenic trioxide. The results showed THL could induce G2/M arrest and apoptosis in NB4 cells. Accordingly, the decrease of cyclin A and B1 were observed in THL-treated cells. The THL-induced apoptosis was accompanied with caspase-3 activation and decrease of PML-RAR alpha fusion protein. Moreover, DNA methyltransferase 1 and oncogenic signaling pathways such as Akt/mTOR, Stat3 and ERK were also down-regulated by THL. By using ethyl acetate extraction and silica gel chromatography, an active fraction of THL named as EAS5 was isolated. At about 0.5-1% of the dose of THL, EAS5 appeared to have most of THL-induced multiple molecular targeting effects in NB4 cells. Based on the findings of these multi-targeting effects, THL might be regarding as a complementary and alternative therapeutic agent for refractory APL.
    Date: 2011-08
    Relation: Evidence-based Complementary and Alternative Medicine. 2011 Aug;2011:Article ID 984154.
    Link to: http://dx.doi.org/10.1093/ecam/nep165
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000293646500001
    Cited Times(Scopus): http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=79953283135
    Appears in Collections:[Shuang-En Chuang] Periodical Articles
    [Gi-Ming Lai(2004-2008)] Periodical Articles

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