國家衛生研究院 NHRI:Item 3990099045/6054

NHRI > National Institute of Infectious Diseases and Vaccinology > Joe Yen-Hung Chow > Periodical Articles > Item 3990099045/6054

Please use this identifier to cite or link to this item: http://ir.nhri.org.tw/handle/3990099045/6054

Title:	Immunoprotectivity of HLA-A2 CTL peptides derived from respiratory syncytial virus fusion protein in HLA-A2 transgenic mouse
Authors:	Shao, HY;Lin, YW;Yu, SL;Lin, HY;Chitra, E;Chang, YC;Sia, C;Chong, P;Hsu, MT;Wei, OL;Chow, YH
Contributors:	Division of Vaccine Research and Development
Abstract:	Identification of HLA-restricted CD8+T cell epitopes is important to study RSV-induced immunity and illness. We algorithmically analyzed the sequence of the fusion protein (F) of respiratory syncytial virus (RSV) and generated synthetic peptides that can potentially bind to HLA-A0201. Four out of the twenty-five 9-mer peptides tested: peptides 3 (F33–41), 13 (F214–222), 14 (F273–281), and 23 (F559–567), were found to bind to HLA-A0201 with moderate to high affinity and were capable of inducing IFN-c and IL-2 secretion in lymphocytes from HLA-A*0201 transgenic (HLA-Tg) mice pre-immunized with RSV or recombinant adenovirus expressing RSV F. HLA-Tg mice were immunized with these four peptides and were found to induce both Th1 and CD8+T cell responses in in vitro secondary recall. Effector responses induced by these peptides were observed to confer differential protection against live RSV challenge. These peptides also caused better recovery of body weight loss induced by RSV. A significant reduction of lung viral load was observed in mice immunized with peptide 23, which appeared to enhance the levels of inflammatory chemokines (CCL17, CCL22, and IL-18) but did not increase eosinophil infiltration in the lungs. Whereas, significant reduction of infiltrated eosinophils induced by RSV infection was found in mice pre-immunized with peptide 13. Our results suggest that HLA-A2-restricted epitopes of RSV F protein could be useful for the development of epitope-based RSV vaccine.
Date:	2011-09
Relation:	PLoS ONE. 2011 Sep;6(9):Article number e25500.
Link to:	http://dx.doi.org/10.1371/journal.pone.0025500
JIF/Ranking 2023:	http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=1932-6203&DestApp=IC2JCR
Cited Times(WOS):	https://www.webofscience.com/wos/woscc/full-record/WOS:000295939600031
Cited Times(Scopus):	http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=80054789893
Appears in Collections:	[Joe Yen-Hung Chow] Periodical Articles [Charles Dwo-Yuan Sia(2006-2014)] Periodical Articles [Pele Choi-Sing Chong] Periodical Articles

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