國家衛生研究院 NHRI:Item 3990099045/6065

NHRI > National Institute of Infectious Diseases and Vaccinology > Ih-Jen Su(2002-2015) > Periodical Articles > Item 3990099045/6065

Please use this identifier to cite or link to this item: http://ir.nhri.org.tw/handle/3990099045/6065

Title:	A single nucleotide in stem loop II of 5'-untranslated region contributes to virulence of Enterovirus 71 in mice
Authors:	Yeh, MT;Wang, SW;Yu, CK;Lin, KH;Lei, HY;Su, IJ;Wang, JR
Contributors:	Division of Infectious Diseases
Abstract:	Background: Enterovirus 71 (EV71) has emerged as a neuroinvasive virus responsible for several large outbreaks in the Asia-Pacific region while virulence determinant remains unexplored. Principal Findings: In this report, we investigated increased virulence of unadapted EV71 clinical isolate 237 as compared with isolate 4643 in mice. A fragment 12 nucleotides in length in stem loop (SL) II of 237 59-untranslated region (UTR) visibly reduced survival time and rate in mice was identified by constructing a series of infectious clones harboring chimeric 59-UTR. In cells transfected with bicistronic plasmids, and replicon RNAs, the 12-nt fragment of isolate 237 enhanced translational activities and accelerated replication of subgenomic EV71. Finally, single nucleotide change from cytosine to uridine at base 158 in this short fragment of 59-UTR was proven to reduce viral translation and EV71 virulence in mice. Results collectively indicated a pivotal role of novel virulence determinant C158 on virus translation in vitro and EV71 virulence in vivo. Conclusions: These results presented the first reported virulence determinant in EV71 59-UTR and first position discovered from unadapted isolates.
Date:	2011-11
Relation:	PLoS ONE. 2011 Nov;6(11):Article number e27082.
Link to:	http://dx.doi.org/10.1371/journal.pone.0027082
JIF/Ranking 2023:	http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=1932-6203&DestApp=IC2JCR
Cited Times(WOS):	https://www.webofscience.com/wos/woscc/full-record/WOS:000297150900050
Cited Times(Scopus):	http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=80055119062
Appears in Collections:	[Ih-Jen Su(2002-2015)] Periodical Articles

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