國家衛生研究院 NHRI:Item 3990099045/6067

NHRI > National Institute of Infectious Diseases and Vaccinology > Min-Shi Lee > Periodical Articles > Item 3990099045/6067

Please use this identifier to cite or link to this item: http://ir.nhri.org.tw/handle/3990099045/6067

Title:	Adaptation of high-growth influenza H5N1 vaccine virus in vero cells: Implications for pandemic preparedness
Authors:	Tseng, YF;Hu, AY;Huang, ML;Yeh, WZ;Weng, TC;Chen, YS;Chong, P;Lee, MS
Contributors:	Division of Infectious Diseases;Division of Vaccine Research and Development
Abstract:	Current egg-based influenza vaccine production technology can't promptly meet the global demand during an influenza pandemic as shown in the 2009 H1N1 pandemic. Moreover, its manufacturing capacity would be vulnerable during pandemics caused by highly pathogenic avian influenza viruses. Therefore, vaccine production using mammalian cell technology is becoming attractive. Current influenza H5N1 vaccine strain (NIBRG-14), a reassortant virus between A/Vietnam/1194/2004 (H5N1) virus and egg-adapted high-growth A/PR/8/1934 virus, could grow efficiently in eggs and MDCK cells but not Vero cells which is the most popular cell line for manufacturing human vaccines. After serial passages and plaque purifications of the NIBRG-14 vaccine virus in Vero cells, one high-growth virus strain (Vero-15) was generated and can grow over 10(8) TCID(50)/ml. In conclusion, one high-growth H5N1 vaccine virus was generated in Vero cells, which can be used to manufacture influenza H5N1 vaccines and prepare reassortant vaccine viruses for other influenza A subtypes.
Date:	2011-10
Relation:	PLoS ONE. 2011 OCT;6(10):Article number e24057.
Link to:	http://dx.doi.org/10.1371/journal.pone.0024057
JIF/Ranking 2023:	http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=1932-6203&DestApp=IC2JCR
Cited Times(WOS):	https://www.webofscience.com/wos/woscc/full-record/WOS:000295978600001
Cited Times(Scopus):	http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=80054748686
Appears in Collections:	[Min-Shi Lee] Periodical Articles [Pele Choi-Sing Chong] Periodical Articles [Yung-Chih Hu] Periodical Articles

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