國家衛生研究院 NHRI:Item 3990099045/6296
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    题名: First worldwide proficiency study on variable-number tandem-repeat typing of Mycobacterium tuberculosis complex strains
    作者: De Beer, JL;Kremer, K;Kodmon, C;Supply, P;Van Soolingen, D;Alexander, D;Augustynowicz-Kope'c, E;Bidovec-Stojkovic, U;Boeree, MJ;Brown, T;Cirillo, DM;Cruz, L;David, S;Diaz, R;Dou, HY;Evans, JT;Fauville-Dufaux, M;Fitzgibbon, MM;Garci­a De Viedma, D;Globan, M;Groenheit, R;Haanpera-Heikkine, M;Indra, A;Kam, KM;Kramer, R;Mei, S;Niemann, S;Obrovac, M;Rasmussen, EM;Refregier, G;Robledo, JA;Samper, S;Sharma, MK;Sougakoff, W;Stakenas, P;Stavrum, R;Suffys, PN;Trenkler, J;Wada, T;Warren, RM
    贡献者: Division of Infectious Diseases
    摘要: Although variable-number tandem-repeat (VNTR) typing has gained recognition as the new standard for the DNA fingerprinting of Mycobacterium tuberculosis complex (MTBC) isolates, external quality control programs have not yet been developed. Therefore, we organized the first multicenter proficiency study on 24-locus VNTR typing. Sets of 30 DNAs of MTBC strains, including 10 duplicate DNA samples, were distributed among 37 participating laboratories in 30 different countries worldwide. Twenty-four laboratories used an in-house-adapted method with fragment sizing by gel electrophoresis or an automated DNA analyzer, nine laboratories used a commercially available kit, and four laboratories used other methods. The intra- and interlaboratory reproducibilities of VNTR typing varied from 0% to 100%, with averages of 72% and 60%, respectively. Twenty of the 37 laboratories failed to amplify particular VNTR loci; if these missing results were ignored, the number of laboratories with 100% interlaboratory reproducibility increased from 1 to 5. The average interlaboratory reproducibility of VNTR typing using a commercial kit was better (88%) than that of in-house-adapted methods using a DNA analyzer (70%) or gel electrophoresis (50%). Eleven laboratories using in-house-adapted manual typing or automated typing scored inter- and intralaboratory reproducibilities of 80% or higher, which suggests that these approaches can be used in a reliable way. In conclusion, this first multicenter study has documented the worldwide quality of VNTR typing of MTBC strains and highlights the importance of international quality control to improve genotyping in the future.
    日期: 2012-03
    關聯: Journal of Clinical Microbiology. 2012 Mar;50(3):662-669.
    Link to: http://dx.doi.org/10.1128/jcm.00607-11
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0095-1137&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000300997800020
    Cited Times(Scopus): http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84857386498
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