 |
English
|
正體中文
|
简体中文
|
Items with full text/Total items : 12145/12927 (94%)
Visitors : 856364
Online Users : 522
|
|
|
Loading...
|
Please use this identifier to cite or link to this item:
http://ir.nhri.org.tw/handle/3990099045/6404
|
| Title: | Identification of protein domains on major pilin MrkA that affects the mechanical properties of Klebsiella pneumoniae type 3 fimbriae |
| Authors: | Chan, CH;Chen, FJ;Huang, YJ;Chen, SY;Liu, KL;Wang, ZC;Peng, HL;Yew, TR;Liu, CH;Liou, GG;Hsu, KY;Chang, HY;Hsu, L |
| Contributors: | Institute of Molecular and Genomic Medicine |
| Abstract: | The Klebsiella pneumoniae type 3 fimbriae are mainly composed of MrkA pilins that assemble into a helixlike filament. This study determined the biomechanical properties of the fimbriae and analyzed 11 site-directed MrkA mutants to identify domains that are critical for the properties. Escherichia coli strains expressing type 3 fimbriae with an Ala substitution at either F34, V45, C87, G189, T196, or Y197 resulted in a significant reduction in biofilm formation. The E. coli strain expressing MrkAG189A remained capable of producing a normal number of fimbriae. Although F34A, V45A, T196A, and Y197A substitutions expressed on E. coli strains produced sparse quantities of fimbriae, no fimbriae were observed on the cells expressing MrkAC87A. Further investigations of the mechanical properties of the MrkAG189A fimbriae with optical tweezers revealed that, unlike the wild-type fimbriae, the uncoiling force for MrkAG189A fimbriae was not constant. The MrkAG189A fimbriae also exhibited a lower enthalpy in the differential scanning calorimetry analysis. Together, these findings indicate that the mutant fimbriae are less stable than the wild-type. This study has demonstrated that the C-terminal β strands of MrkA are required for the assembly and structural stability of fimbriae. |
| Date: | 2012-05 |
| Relation: | Langmuir. 2012 May;28(19):7428-7435. |
| Link to: | http://dx.doi.org/10.1021/la300224w |
| JIF/Ranking 2023: | http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0743-7463&DestApp=IC2JCR |
| Cited Times(WOS): | https://www.webofscience.com/wos/woscc/full-record/WOS:000303969000014 |
| Cited Times(Scopus): | http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84861168749 |
| Appears in Collections: | [劉淦光(2006-2014)] 期刊論文
|
Files in This Item:
| File |
Description |
Size | Format | |
|---|
| SCP84861168749.pdf | | 497Kb | Adobe PDF | 791 | View/Open |
|
All items in NHRI are protected by copyright, with all rights reserved.
|
