國家衛生研究院 NHRI:Item 3990099045/6427
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    Please use this identifier to cite or link to this item: http://ir.nhri.org.tw/handle/3990099045/6427


    Title: Neural transmembrane protease and endothelial Gs-protein activation in cell contact-dependent signaling between neural stem/progenitor cells and brain endothelial cells
    Other Titles: Neural Transmembrane Protease and Endothelial G(s) Protein Activation in Cell Contact-dependent Signaling between Neural Stem/Progenitor Cells and Brain Endothelial Cells
    Authors: Tung, HH;Lee, SL
    Contributors: Institute of Cellular and Systems Medicine
    Abstract: Vasculature is an important component of the neural stem cell niche in brain. It regulates neural stem/progenitor (NS/P) cell self-renewal, differentiation and migration. In the neurogenic niches of adult brain, NS/P cells lie close to blood vessels and proliferating NS/P cells frequently contact the vasculature. In the present study we showed that NS/P cells in co-culture with brain endothelial (bEND) cells activated endothelial G-proteins and p38 mitogenic activated protein kinase (p38MAPK), and stimulated cytokine/chemokine expression. These NS/P cell-induced endothelial responses took place during NS/P cell and bEND cell direct contact and were critically dependent on the expression of the type II transmembrane serine protease matriptase (MTP) by NS/P cells, since knocking down of MTP in NS/P cells impaired and re-expression of MTP restored their ability to induce endothelial cytokine/chemokine expression, p38MAPK or G-protein activation. Cholera toxin blocked NS/P cell-induced endothelial responses suggesting the endothelial G-protein activated by NS/P MTP is in the Gs subfamily. Addition of p38MAPK inhibitor impaired NS/P cell-induced endothelial cytokine/ chemokine expression. The known G-protein coupled receptor substrate of MTP, protease activated receptor 2, was not involved in this system. These results revealed a novel signaling pathway in neural stem cell vascular niches that is mediated by neural MTP and endothelial Gs-protein signaling at the cell-cell interface. This is the first report of direct cell-cell signaling between NS/P and bEND cells.
    Date: 2012-06
    Relation: Journal of Biological Chemistry. 2012 Jun;287(27):22497-22508.
    Link to: http://dx.doi.org/10.1074/jbc.M111.330589
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=1083-351X&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000306495000008
    Cited Times(Scopus): http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84863321968
    Appears in Collections:[Sheau-Ling Lee] Periodical Articles

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