國家衛生研究院 NHRI:Item 3990099045/6454
English  |  正體中文  |  简体中文  |  全文笔数/总笔数 : 12145/12927 (94%)
造访人次 : 914878      在线人数 : 1415
RC Version 6.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
搜寻范围 查询小技巧：
  • 您可在西文检索词汇前后加上"双引号"，以获取较精准的检索结果
  • 若欲以作者姓名搜寻，建议至进阶搜寻限定作者字段，可获得较完整数据
    •  进阶搜寻
    主页 ‧ 登入 ‧ 上传 ‧ 说明 ‧ 关于NHRI ‧ 管理 到手机版


    jsp.display-item.identifier=請使用永久網址來引用或連結此文件: http://ir.nhri.org.tw/handle/3990099045/6454


    题名: Role of the Aryl-hydrocarbon Receptor (AhR)-ligand axis in mast cells
    作者: Zhou, Y;Tung, H;Tsai, Y;Hsu, S;Plunkett, B;Vonakis, BM;Huang, S
    贡献者: Division of Environmental Health and Occupational Medicine
    摘要: RATIONALE: The significance of AhR signaling in immune regulation has been suggested, but its role in mast cells remains unknown. We hypothesized that AhR and its ligands may affect mast cells function and play an important role in the regulation of allergic diseases. METHODS: Mouse bone marrow-derived mast cells (BMMCs) were treated with several known AhR ligands including TCDD and FICZ. The levels of cytokines and mediators release in activated mast cells by IgE/Ag cross-linkage were analyzed. The in vivo effect was tested by passive cutaneous anaphylaxis (PCA). Reactive oxygen species (ROS) and intra-cellular calcium were detected by Flow cytometry. ERK and PKC phosphorylation was detected by Western blotting. RESULTS: Exposure of BMMCs to low-doses ( < 10 nM) of AhR ligands significantly increased the levels of IL-13, degranulation, LTC 4 , and PCA response. Mechanistically, exposure of the cells to AhR ligands resulted in significantly increased levels of intracellular calcium and ROS, concomi-tant with enhanced activation of several signaling pathways involving ERK and PKC. Notably, the functional effects of AhR ligands could be re-versed by the addition of an antioxidant, N-acetylcysteine, and calcium channel blockers. In addition, when BMMCs from AhR-null mice were an-alyzed, significant reductions in the numbers of BMMCs and in the levels of IL-3-induced proliferation were noted, as compared to those seen in wild-type mice. Mast cell deficiency was confirmed in various tissues and peritoneal cavity of AhR-null mice. CONCLUSIONS: These results thus provide evidence supporting critical roles of AhR signaling in maintaining the homeostasis, maturation and optimal activation of mast cells.
    日期: 2012-02
    關聯: Journal of Allergy and Clinical Immunology. 2012 Feb;129(2 Suppl):AB121.
    Link to: http://dx.doi.org/10.1016/j.jaci.2011.12.581
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0091-6749&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000301133400458
    显示于类别:[黃嘯谷] 會議論文/會議摘要

    文件中的档案:

    档案 描述 大小格式浏览次数
    ISI000301133400458.pdf39KbAdobe PDF566检视/开启


    在NHRI中所有的数据项都受到原著作权保护.

    TAIR相关文章

    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - 回馈