國家衛生研究院 NHRI:Item 3990099045/6596
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    题名: Cholaminchloride hydrochloride-cationized gelatin/calcium-phosphate nanoparticles as gene carriers for transgenic chicken production
    作者: Huang, JY;Liu, CC;Yen, KC;Kang, PL;Sadhasivam, S;Lin, FH
    贡献者: Division of Medical Engineering Research
    摘要: Currently, transgenic chickens are a popular method for pharmaceutical protein production. In this study, we employed cationized gelatin/calcium phosphate nanoparticles (ca-GCaPs) with surface modification by cholaminchloride hydrochloride as gene carriers to produce transgenic chickens. To evaluate the transfection efficiency, a plasmid (pEGFP-C1) was encapsulated in the ca-GCaPs for HeLa cells transfection. The ca-GCaPs were applied as gene carriers by direct injection into the area opaca of the chicken embryo blastodisc to produce the transgenic chicken. The particle size of the ca-GCaPs was 350 nm and the zeta potential was +15 mV. Plasmids encapsulated in the ca-GCaPs prevented from DNase I degradation. Based on biocompatibility analysis, the ca-GCaPs produce higher cell viability and less cytotoxicity compared to the commercially available product Lipofectamine™ 2000. The in vitro study indicated that the transfection rate using ca-GCaPs was higher than using cationized gelatin nanoparticles (ca-GPs) because calcium phosphate can provide osmotic pressure to break down lysosomes. In the in vivo study, the egg hatch rate was 100% after ca-GCaPs transfection, and green fluorescence protein expression could be observed in chicken tissue on the fourth day after transfection.
    日期: 2012-12
    關聯: Process Biochemistry. 2012 Dec;47(12):1919-1925.
    Link to: http://dx.doi.org/10.1016/j.procbio.2012.06.023
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=1359-5113&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000313851700028
    Cited Times(Scopus): http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84870822282
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