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http://ir.nhri.org.tw/handle/3990099045/6909
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| Title: | Detection of caga expression in gastric mucosa-associated lymphoid tissue lymphoma-biologic significance and clinical implication |
| Authors: | Kuo, S;Chen, L;Lin, C;Wu, M;Hsu, P;Tzeng, Y;Tsai, H;Wang, H;Yeh, K;Cheng, A |
| Contributors: | National Institute of Cancer Research |
| Abstract: | Background: We recently reported that a direct contact of Helicobacter pylori (HP) with B cells resulted in CagA translocation into the cells (Cancer Res 2010;70:5740-8). The translocated CagA further activates extracellular signal-regulated kinase (ERK) and p38 mitogen-activated protein kinase (MAPK), and up-regulates the expressions of Bcl-2 and Bcl-xL. In this study, we sought to verify if CagA exists in the malignant B cells of gastric mucosa-associated lymphoid tissue (MALT) lymphoma. Methods: Expression of CagA protein, phospho-SHP-2, phospho-ERK, phospho-p38MAPK, Bcl-2 and Bcl-xL in a series of 26 gastric MALT lymphoma was determined by immunohistochemistry. Western blot analysis was done to confirm immunohistochemical detection of CagA and biopsies from non-gastric MALT lymphoma served as negative controls. The association of CagA expression and the tumor response to HP-eradication (HPE) therapy was evaluated in 77 stage IE/IIE1 low-grade gastric MALT lymphoma patients. Results: The expression of CagA was detected in 35 (45.5%) of 77 patients. CagA expression was closely associated with the expression phospho-SHP-2 (P = .016), phospho-ERK (P < .001), phospho-p38MAPK (P = 0.014), Bcl-2 (P = 0.009), and Bcl-xL (P = 0.008). CagA expression was detected in 30 (69.8%) of 43 HP-dependent cases, and in 5 (14.7%) of 34 HP-independent cases (P < 0.001). Furthermore, patients with CagA expression responded to HPE more rapidly than those without (median time to complete remission, 3.0 months versus 7.0 months, P = 0.032). Conclusion: CagA protein can be translocated into malignant B cells of MALT lymphoma. The expression of CagA in lymphoma cells appears to be biologically and clinically significant. |
| Date: | 2012-09 |
| Relation: | Annals of Oncology. 2012 Sep;23(Suppl. 9):351. |
| Link to: | http://dx.doi.org/10.1093/annonc/mds403 |
| JIF/Ranking 2023: | http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0923-7534&DestApp=IC2JCR |
| Cited Times(WOS): | https://www.webofscience.com/wos/woscc/full-record/WOS:000309409002014 |
| Appears in Collections: | [陳立宗] 會議論文/會議摘要
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| ISI000309409002014.pdf | | 214Kb | Adobe PDF | 352 | View/Open |
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