國家衛生研究院 NHRI:Item 3990099045/6976
English  |  正體中文  |  简体中文  |  全文笔数/总笔数 : 12145/12927 (94%)
造访人次 : 909371      在线人数 : 733
RC Version 6.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
搜寻范围 查询小技巧：
  • 您可在西文检索词汇前后加上"双引号"，以获取较精准的检索结果
  • 若欲以作者姓名搜寻，建议至进阶搜寻限定作者字段，可获得较完整数据
    •  进阶搜寻
    主页 ‧ 登入 ‧ 上传 ‧ 说明 ‧ 关于NHRI ‧ 管理 到手机版


    jsp.display-item.identifier=請使用永久網址來引用或連結此文件: http://ir.nhri.org.tw/handle/3990099045/6976


    题名: Role of histone deacetylases in regulation of NF-E2-related factor 2, kruppel-like factor 2, and cell cycle in vascular endothelial cells in response to disturbed flow
    作者: Chiu, JJ;Lee, DY;Chien, S
    贡献者: Division of Medical Engineering Research
    摘要: Vascular endothelial cells (ECs) are exposed to different patterns of blood flow (disturbed vs. laminar) and the associated shear stresses (oscillatory [OSS] vs. pulsatile [PSS]), which lead to differential EC responses. We investigated the roles of classes I and II histone deacetylases (HDACs, i.e., HDAC-1/2/3 and HDAC-5/7, respectively) in regulating NF-E2-related factor-2 (Nrf2) and Kr?ppel-like factor-2 (KLF2), two important transcription factors that govern many shear-responsive genes, and cell cycle in ECs in response to OSS. Application of OSS (0.5?4 dynes/cm2) to ECs up-regulated both classes I and II HDACs and their nuclear accumulations, whereas PSS (12?4 dynes/cm2) induced phosphorylation-dependent nuclear export of class II HDACs. OSS induced the association of HDAC-1/2/3 with Nrf2 and HDAC-3/5/7 with myocyte enhancer factor-2, and the deacetylations of these HDACs led to down-regulations of the antioxidant gene NAD(P)H quinone oxidoreductase-1 (NQO1) and KLF2 in ECs. Transfecting ECs with HDAC-1/2/3- and HDAC-3/5/7-specific small interfering RNAs eliminated the OSS-induced down-regulations of NQO1 and KLF2, respectively. OSS up-regulated cyclin A and phospho-retinoblastoma and down-regulated p21CIP1 in ECs and hence their proliferation; these effects are mediated by HDAC-1/2/3. The OSS-induced HDAC signaling and EC responses are mediated by phosphatidylinositol 3-kinase/Akt. Immunohistochemical examinations of the experimentally stenosed rat abdominal aorta showed high levels of HDAC-2/3/5 in the EC layer at post-stenotic sites, where OSS occurs. Our findings indicate the importance of different HDACs in regulating endothelial oxidative, inflammatory, and proliferative responses to disturbed flow with OSS.
    日期: 2012-04
    關聯: FASEB Journal. 2012 Apr;26:1129.1.
    Link to: http://www.fasebj.org/cgi/content/meeting_abstract/26/1_MeetingAbstracts/1129.1
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0892-6638&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000310711300689
    显示于类别:[裘正健] 會議論文/會議摘要

    文件中的档案:

    档案 描述 大小格式浏览次数
    ISI000310711300689.pdf40KbAdobe PDF606检视/开启


    在NHRI中所有的数据项都受到原著作权保护.

    TAIR相关文章

    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - 回馈