國家衛生研究院 NHRI:Item 3990099045/7157
English  |  正體中文  |  简体中文  |  全文笔数/总笔数 : 12189/12972 (94%)
造访人次 : 955188      在线人数 : 613
RC Version 6.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
搜寻范围 查询小技巧：
  • 您可在西文检索词汇前后加上"双引号"，以获取较精准的检索结果
  • 若欲以作者姓名搜寻，建议至进阶搜寻限定作者字段，可获得较完整数据
    •  进阶搜寻
    主页 ‧ 登入 ‧ 上传 ‧ 说明 ‧ 关于NHRI ‧ 管理 到手机版


    jsp.display-item.identifier=請使用永久網址來引用或連結此文件: http://ir.nhri.org.tw/handle/3990099045/7157


    题名: Transplantation of human corneal endothelial cells using functional biomaterials: Poly(N-isopropylacrylamide) and gelatin
    作者: Hsu, WM;Chen, KH;Lai, JY;Hsiue, GH
    贡献者: Division of Medical Engineering Research
    摘要: Purpose: To evaluate the feasibility of human corneal endothelial cell (HCEC) transplantation by harvesting the HCEC sheet on a thermoresponsive surface, delivering it with a gelatin disc, and testing it in a rabbit model. Methods: Cultivated human adult HCECs labeled with a red fluorescent dye (PKH26) were seeded on a poly(N-isopropylacrylamide) (PNIPAAM)-grafted surface culture dish at 37°C. After reaching confluence, the HCEC monolayer was detached by reducing the incubation temperature to 20°C and immediately delivered by means of a 7-mm gelatin disc to the rabbit's cornea denuded with endothelial cells (HCEC group, n = 8). The morphology, viability, pump, and barrier functions of the harvested HCEC were evaluated. Traumatized rabbit corneas with only the gelatin disc graft (gelatin disc group, n = 4) and without any transplantation (wound group, n = 4) were the sham controls. Surgical corneas of each group underwent histological and clinical evaluations including corneal thickness, intraocular pressure (IOP), and corneal clarity at different time points during a follow-up period of 12 weeks. Results: Cell morphology, viability, densities, Na+/K+ ATPase, and zonula occluden-1 (ZO-1) of the cultivated HCEC monolayer were similar with those of native HCECs. After endothelial removal, corneas of three groups turned severely edematous and opaque. In the HCEC group, the clarity of cornea recovered within 2 weeks with a corneal thickness of 552 ± 18 μm, which was significantly less than those (>1,000 μm) of the control groups (p < 0.05). Histological examinations showed that the PKH26-labeled HCECs were spread over the Descemet's membrane with tight junction formation in the HCEC group, but none in the control group. The postoperative IOP of all three groups was within normal limits. Conclusion:This study provides a novel strategy for reconstruction of corneal endothelial cells using cultured adult HCECs and functional biomaterials including PNIPAAM and gelatin.
    日期: 2013-04
    關聯: Journal of Experimental and Clinical Medicine. 2013 Apr;5(2):56-64.
    Link to: http://dx.doi.org/10.1016/j.jecm.2013.02.001
    Cited Times(Scopus): http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84876109235
    显示于类别:[其他] 期刊論文

    文件中的档案:

    档案 描述 大小格式浏览次数
    SCP84875130902.pdf1819KbAdobe PDF441检视/开启


    在NHRI中所有的数据项都受到原著作权保护.

    TAIR相关文章

    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - 回馈