國家衛生研究院 NHRI:Item 3990099045/7321
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    Please use this identifier to cite or link to this item: http://ir.nhri.org.tw/handle/3990099045/7321


    Title: Dissecting the EGFR-PI3K-AKT pathway in oral cancer highlights the role of the EGFR variant III and its clinical relevance
    Authors: Chang, KY;Tsai, SY;Chen, SH;Tsou, HH;Yen, CJ;Liu, KJ;Fang, HL;Wu, HC;Chuang, BF;Chou, SW;Tang, CK;Liu, SY;Lu, PJ;Yen, CY;Chang, JY
    Contributors: National Institute of Cancer Research;Division of Biostatistics and Bioinformatics
    Abstract: BACKGROUND: Dysregulated epidermal growth factor receptor (EGFR)-phosphoinositide-3-kinase (PI3K)-AKT signaling is considered pivotal for oral cancer, and the pathway is a potential candidate for therapeutic targeting. RESULTS: A total of 108 archival samples which were from surgically resected oral cancer were examined. Immunohistochemical staining showed the protein expression of membranous wild-type EGFR and cytoplasmic phosphorylated AKT was detected in 63.9% and 86.9% of the specimens, respectively. In 49.1% of the samples, no phosphatase and tensin homolog (PTEN) expression was detected. With regard to the EGFR variant III (EGFRvIII), 75.0% of the samples showed positive expression for moderate to severe staining, 31.5% of which had high expression levels. Real-time polymerase chain reaction assays for gene copy number assessment of PIK3CA revealed that 24.8% of the samples had alterations, and of EGFR showed that 49.0% had amplification. Direct sequencing of PIK3CA gene showed 2.3% of the samples had a hotspot point mutation. Statistical assessment showed the expression of the EGFRvIII correlated with the T classification and TNM stage. The Kaplan-Meier analyses for patient survival showed that the individual status of phosphorylated AKT and EGFRvIII led to significant differences in survival outcome. The multivariate analysis indicated that phosphorylated AKT, EGFRvIII expression and disease stage were patient survival determinants. CONCLUSIONS: Aberrations in the EGFR-PI3K-AKT pathway were frequently found in oral cancers. EGFRvIII and phosphorylated AKT were predictors for the patient survival and clinical outcome.
    Date: 2013-06-27
    Relation: Journal of Biomedical Science. 2013 Jun 27;20(1):Article number 43.
    Link to: http://dx.doi.org/10.1186/1423-0127-20-43
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=1021-7770&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000321780900001
    Cited Times(Scopus): http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84879456417
    Appears in Collections:[Kwang-Yu Chang] Periodical Articles
    [Ko-Jiunn Liu] Periodical Articles
    [Jang-Yang Chang] Periodical Articles
    [Hsiao-Hui Sophie Tsou] Periodical Articles
    [Shang-Hung Chen] Periodical Articles

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