國家衛生研究院 NHRI:Item 3990099045/7406
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    题名: Surface alpha-enolase promotes extracellular matrix degradation and tumor metastasis and represents a new therapeutic target
    其它题名: Surface α-enolase promotes extracellular matrix degradation and tumor metastasis and represents a new therapeutic target
    作者: Hsiao, KC;Shih, NY;Fang, HL;Huang, TS;Kuo, CC;Chu, PY;Hung, YM;Chou, SW;Yang, YY;Chang, GC;Liu, KJ
    贡献者: National Institute of Cancer Research
    摘要: In previous research, we found α-enolase to be inversely correlated with progression-free and overall survival in lung cancer patients and detected α-enolase on the surface of lung cancer cells. Based on these findings, we hypothesized that surface α-enolase has a significant role in cancer metastasis and tested this hypothesis in the current study. We found that α-enolase was co-immunoprecipitated with urokinase-type plasminogen activator, urokinase-type plasminogen activator receptor, and plasminogen in lung cancer cells and interacted with these proteins in a cell-free dot blotting assay, which can be interrupted by α-enolase-specific antibody. α-Enolase in lung cancer cells co-localized with these proteins and was present at the site of pericellular degradation of extracellular matrix components. Treatment with antibody against α-enolase in vitro suppressed cell-associated plasminogen and matrix metalloproteinase activation, collagen and gelatin degradation, and cell invasion. Examination of the effect of treatment with shRNA plasmids revealed that down regulation of α-enolase decreases extracellular matrix degradation by and the invasion capacity of lung cancer cells. Adoptive transfer of α-enolase-specific antibody to mice resulted in accumulation of antibody in subcutaneous tumor and inhibited the formation of tumor metastasis in lung and bone. This study demonstrated that surface α-enolase promotes extracellular matrix degradation and invasion of cancer cells and that targeting surface α-enolase is a promising approach to suppress tumor metastasis.
    日期: 2013-07-19
    關聯: PLoS ONE. 2013 Jul 19;8(7):Article number e69354.
    Link to: http://dx.doi.org/10.1371/journal.pone.0069354
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=1932-6203&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000322391400051
    Cited Times(Scopus): http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84880429753
    显示于类别:[劉柯俊] 期刊論文
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