國家衛生研究院 NHRI:Item 3990099045/7563
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    Please use this identifier to cite or link to this item: http://ir.nhri.org.tw/handle/3990099045/7563


    Title: A mutation of the Col2alpha1 gene (G1170S) alters the transgenic murine phenotype and cartilage matrix homeostasis
    Authors: Yang, RC;Chen, MH;Chen, PY;Chen, CY;Tsai, SF;Cheng, CK;Sun, JS
    Contributors: Institute of Molecular and Genomic Medicine
    Abstract: Background/Purpose Genomic studies have revealed that there is a significant association between a point mutation of the human Col2A1 gene (G1170S) and several hip disorders. The purpose of the study was to explore the phenotype and altered cartilage matrix homeostasis of transgenic mice carrying this mutated Col2a1 gene. Methods Wild-type and transgenic mice were used as the control and study groups, respectively. Body weight measurement, radiographic analysis, and histological analysis of the mice were carried out to describe differences between the wild-type and transgenic mice at different ages. Cartilage metabolism studies were also carried out, including an MTT assay of cellular proliferation and nitric oxide and glycosaminoglycan assays. Allelic expression levels of the mutant A allele and the normal G allele were established by TaqMan assay. Cytokine and protease gene expression were measured. Results Transgenic mice had a lower mean body weight, a deformed skeletal structure, and abnormal cartilage histomorphology. Chondrocyte proliferation was significantly compromised and this was linked to significantly higher NO secretion and less soluble glycosaminoglycan formation. TNF-α and IL-1β gene expression was significantly upregulated, while MMP-13 gene expression was significantly downregulated. Conclusion The mutant G1170S Col2a1 gene in mice clearly alters the transgenic murine phenotype and cartilage matrix homeostasis.
    Date: 2014-11
    Relation: Journal of the Formosan Medical Association. 2014 Nov;113(11):803-812.
    Link to: http://dx.doi.org/10.1016/j.jfma.2013.09.015
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0929-6646&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000345557100005
    Cited Times(Scopus): http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84922059171
    Appears in Collections:[Shih-Feng Tsai] Periodical Articles

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