國家衛生研究院 NHRI:Item 3990099045/7590
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    题名: KPC-2-encoding plasmids from Escherichia coli and Klebsiella pneumoniae in Taiwan
    作者: Chen, YT;Lin, JC;Fung, CP;Lu, PL;Chuang, YC;Wu, TL;Siu, LK
    贡献者: Division of Infectious Diseases;Institute of Molecular and Genomic Medicine
    摘要: OBJECTIVES: Two plasmids carrying blaKPC-2 isolated from carbapenem-resistant Escherichia coli (CR-EC) and carbapenem-resistant Klebsiella pneumoniae (CR-KP), respectively, were completely sequenced. The CR-KP strain was selected from an outbreak in 2012, and the CR-EC strain was the first blaKPC-2-carrying E. coli identified in the same carbapenem resistance monitoring programme in Taiwan. METHODS: Antimicrobial susceptibility tests, multilocus sequence typing (MLST) and the conjugal transfer of plasmids were performed. Complete sequencing of the plasmids was performed using a shotgun approach. RESULTS: The CR-EC and CR-KP strains in this study were determined to be ST410 and ST11, respectively, by MLST. From CR-EC, we identified a 145 kb conjugative plasmid that carries blaKPC-2, blaCMY-2, blaCTX-M-3 and blaTEM-1. The plasmid is a chimera composed of three regions related to IncI, IncN and RepFIC replicons. From CR-KP, we identified an 86.5 kb plasmid, pKPC-LK30, which carries blaKPC-2 and blaSHV-11. The plasmid is very similar to two blaKPC-2-carrying IncFIIK plasmids, but lacks one of the replication origins and cannot conjugate. CONCLUSIONS: The differences in cross-species transferability of the two plasmids can be explained by genetic differences between their backbones and could have resulted in the confined blaKPC-2-carrying CR-KP outbreak in Taiwan. Plasmid pKPC-LKEc is the first blaKPC-2-carrying plasmid identified from CR-EC in Taiwan. With relatively high transferability it should be closely monitored.
    日期: 2014-03
    關聯: Journal of Antimicrobial Chemotherapy. 2014 Mar;69(3):628-631.
    Link to: http://dx.doi.org/10.1093/jac/dkt409
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0305-7453&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000331827200009
    Cited Times(Scopus): http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84894053616
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