國家衛生研究院 NHRI:Item 3990099045/7637
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    题名: Human dendritic cell and natural killer cell activation by recombinant hemagglutinin proteins of H1N1 influenza A virus
    作者: Lee, YC;Lin, SJ;Wu, SC;Hsiao, HS;Lee, PT;Liu, WC;Yeh, KW;Huang, JL
    贡献者: Division of Vaccine Research and Development
    摘要: Background: Influenza A virus (flu-A) can cause lethal respiratory tract infections, as observed in a pandemic H1N1 outbreak in 2009. Vaccination using inactivated virus is advised to prevent severe flu-A infections with questionable efficacy. Recombinant hemagglutinin proteins (rHA) may prove to be an effective way of inducing protective immunity against flu-A infection. Dendritic cells and natural killer cells are players of innate immunity and play an important role in developing subsequent adaptive immunity against flu-A. Methods: We compare the effect of the whole flu-A virus and the rHAs from two different H1N1 virus (A/WSN/33,/Texas/05/2009) on the maturation and activation of human monocyte-derived dendritic cells (MoDCs) and beads-purified natural killer (NK) cells. Results:(i) rHA of flu-A induced maturation and activation of MoDCs as effective as whole Influenza A/WSN/33 virus, as demonstrated by increased CD80,CD83, CD86 expression; (ii) rHA induce lesser degree of apoptosis of DCs, compared to the whole influenza A/WSN/33 virus ( P < 0.05); (iii) rHA treated DC produced less cytokines (TNF-a , INF-a ) than did flu-A infected DCs (P<0.01). As to NKcells, we established that (i) Both flu-A and rHA resulted in apoptosis of NK cells; (ii) flu-A but not rHA induced down-regulation of NKp46 expression on NK cells; (iii) Natural killer cytotocixity was suppressed by flu-A (P<0.05) but not rHAs.(P>0.05).Conclusion(s):Taken together, rHA andflu-A showed differential effect on maturation and activation of human dendritic cells and NK cells. These findings may provide useful information for the development of more effective influenza vaccines.
    日期: 2013-11
    關聯: Allergy. 2013 Nov;68(Suppl. S98):7.
    Link to: http://dx.doi.org/10.1111/all.12247
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0105-4538&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000325897100024
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