國家衛生研究院 NHRI:Item 3990099045/7833
English  |  正體中文  |  简体中文  |  全文筆數/總筆數 : 12145/12927 (94%)
造訪人次 : 857790      線上人數 : 819
RC Version 6.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
搜尋範圍 查詢小技巧：
  • 您可在西文檢索詞彙前後加上"雙引號"，以獲取較精準的檢索結果
  • 若欲以作者姓名搜尋，建議至進階搜尋限定作者欄位，可獲得較完整資料
    •  進階搜尋
    主頁 ‧ 登入 ‧ 上傳 ‧ 說明 ‧ 關於NHRI ‧ 管理 到手機版


    請使用永久網址來引用或連結此文件: http://ir.nhri.org.tw/handle/3990099045/7833


    題名: The inducible Nitric-oxide Synthase (iNOS)/Src axis mediates toll-like receptor 3 tyrosine 759 phosphorylation and enhances its signal transduction, leading to interferon-beta synthesis in macrophages
    其他題名: The inducible Nitric-oxide Synthase (iNOS)/Src axis mediates toll-like receptor 3 tyrosine 759 phosphorylation and enhances its signal transduction, leading to interferon-β synthesis in macrophages
    作者: Hsieh, MY;Chang, MY;Chen, YJ;Li, YK;Chuang, TH;Yu, GY;Cheung, CH;Chen, HC;Maa, MC;Leu, TH
    貢獻者: Immunology Research Center;Division of Infectious Diseases
    摘要: Double-stranded RNA (dsRNA) induces phosphorylation of Toll-like receptor 3 (TLR3) at Tyrosine 759 (Y759) and subsequently triggers signaling pathways to promote interferon-beta (IFN-beta) production. In this study, we found that dsRNA-stimulation induces biphasic TLR3 Y759-phosphorylation in macrophages. In addition to the immediate TLR3 Y759-phosphorylation, we identified a second wave of Y759-phosphorylation accompanied with the increase of both Src and ifn-beta transcription in the later phase of dsRNA-stimulation. Interestingly, Src phosphorylated TLR3 Y759 in vitro and in vivo. However, knockdown of Src abolished the late phase of TLR3 Y759-phosphorlyation and decreased the nuclear accumulation of interferon regulatory factors (IRFs) 3 and 7 and IFN-beta production. Re-introduction of Src restored all these molecular changes. Notably, via down-regulation of Src, dsRNA-elicited TLR3 Y759-phosphorylation, the nuclear accumulation of IRF3/IRF7, and IFN-beta generation were inhibited in inducible nitric oxide synthase (iNOS)-null macrophages. TLR3 knockdown destabilized Src and reduced the nuclear level of IRF3/IRF7 and IFN-beta production in macrophages exposed to LPS (a TLR4 ligand known to induce Src and IFN-beta expression). Ectopic expression of wild type TLR3, but not its 759-phenylalanine mutant, restored Src activity and ifn-beta transcription. Taken together, these results suggested an essential role of iNOS/Src/TLR3 axis in IFN-beta production in macrophages.
    日期: 2014-03
    關聯: Journal of Biological Chemistry. 2014 Mar;289(13):9208-9220.
    Link to: http://dx.doi.org/10.1074/jbc.M113.508663
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=1083-351X&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000333472100039
    Cited Times(Scopus): http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84897415714
    顯示於類別:[莊宗顯] 期刊論文
    [余冠儀] 期刊論文

    文件中的檔案:

    檔案 描述 大小格式瀏覽次數
    PB2014022503.pdf2728KbAdobe PDF620檢視/開啟


    在NHRI中所有的資料項目都受到原著作權保護.

    TAIR相關文章

    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - 回饋