國家衛生研究院 NHRI:Item 3990099045/7846
English  |  正體中文  |  简体中文  |  全文筆數/總筆數 : 12145/12927 (94%)
造訪人次 : 915555      線上人數 : 1249
RC Version 6.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
搜尋範圍 查詢小技巧：
  • 您可在西文檢索詞彙前後加上"雙引號"，以獲取較精準的檢索結果
  • 若欲以作者姓名搜尋，建議至進階搜尋限定作者欄位，可獲得較完整資料
    •  進階搜尋
    主頁 ‧ 登入 ‧ 上傳 ‧ 說明 ‧ 關於NHRI ‧ 管理 到手機版


    請使用永久網址來引用或連結此文件: http://ir.nhri.org.tw/handle/3990099045/7846


    題名: Protein transduction in human cellsmediated by arginine-richcell-penetrating peptides in mixedcovalent and noncovalent manners
    作者: Liu, BR;Chan, MH;Chen, HH;Huang, YW;Lee, HJ
    貢獻者: Center for Neuropsychiatric Research
    摘要: Cell-penetrating peptides (CPPs) are small peptides with a highcontent of basic amino acid residues. They possess the ability to translocate through the plasma membrane and facilitate exogenous cargodelivery into living cells. In this chapter, we demonstrate that argininerichCPPs are able to not only traverse cellular membranes by themselves,but also carry macromolecules into human A549 lung carcinoma cells inmixed covalent and noncovalent manners. This special macromoleculardelivery system was named as mixed covalent and noncovalent proteintransductions (CNPT). We found that cells treated with nona-arginine(R9)-red fluorescent protein (RFP) fusion protein mixed with greenfluorescent protein (GFP), referred to as R9-RFP/GFP complexes, exhibitboth red and green fluorescent images. Cells treated with R9-GFP fusionprotein mixed with RFP, denoted as R9-GFP/RFP complexes, emittedgreen and red fluorescence, vice versa. Furthermore, mechanistic studiesrevealed that the cellular uptake mechanism of CNPT may involve acombination of multiple internalization pathways. Therefore, applicationsof this binary CNPT system may provide an efficient tool for delivery ofmultiple proteins in bioscience and clinical research.
    日期: 2013-05
    關聯: Macromolecular Chemistry: New Research. 2013 May 17:69-82.
    Link to: https://www.novapublishers.com/catalog/product_info.php?products_id=43405
    Cited Times(Scopus): http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84892866816
    顯示於類別:[陳慧諴] 圖書

    文件中的檔案:

    沒有與此文件相關的檔案.



    在NHRI中所有的資料項目都受到原著作權保護.

    TAIR相關文章

    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - 回饋