國家衛生研究院 NHRI:Item 3990099045/7907
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    Please use this identifier to cite or link to this item: http://ir.nhri.org.tw/handle/3990099045/7907


    Title: MicroRNA-149 targets GIT1 to suppress integrin signaling and breast cancer metastasis
    Authors: Chan, SH;Huang, WC;Chang, JW;Chang, KJ;Kuo, WH;Wang, MY;Lin, KY;Uen, YH;Hou, MF;Lin, CM;Jang, TH;Tu, CW;Lee, YR;Lee, YH;Tien, MT;Wang, LH
    Contributors: Institute of Molecular and Genomic Medicine
    Abstract: Metastasis is the predominant cause of death in breast cancer patients. Several lines of evidence have shown that microRNAs (miRs) can have an important role in cancer metastasis. Using isogenic pairs of low and high metastatic lines derived from a human breast cancer line, we have identified miR-149 to be a suppressor of breast cancer cell invasion and metastasis. We also identified GIT1 (G-protein-coupled receptor kinase-interacting protein 1) as a direct target of miR-149. Knockdown of GIT1 reduced migration/invasion and metastasis of highly invasive cells. Re-expression of GIT1 significantly rescued miR-149-mediated inhibition of cell migration/invasion and metastasis. Expression of miR-149 impaired fibronectin-induced focal adhesion formation and reduced phosphorylation of focal adhesion kinase and paxillin, which could be restored by re-expression of GIT1. Inhibition of GIT1 led to enhanced protein degradation of paxillin and α5β1 integrin via proteasome and lysosome pathways, respectively. Moreover, we found that GIT1 depletion in metastatic breast cancer cells greatly reduced α5β1-integrin-mediated cell adhesion to fibronectin and collagen. Low level of miR-149 and high level of GIT1 was significantly associated with advanced stages of breast cancer, as well as with lymph node metastasis. We conclude that miR-149 suppresses breast cancer cell migration/invasion and metastasis by targeting GIT1, suggesting potential applications of the miR-149-GIT1 pathway in clinical diagnosis and therapeutics.
    Date: 2014-09
    Relation: Oncogene. 2014 Sep;33(36):4496-4507.
    Link to: http://dx.doi.org/10.1038/onc.2014.10
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0950-9232&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000341158800005
    Cited Times(Scopus): http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84895417104
    Appears in Collections:[Lu-Hai Wang] Periodical Articles

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