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http://ir.nhri.org.tw/handle/3990099045/8010
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| Title: | Epstein-Barr virus BALF3 has nuclease activity and mediates mature virion production during the lytic cycle |
| Authors: | Chiu, SH;Wu, MC;Wu, CC;Chen, YC;Lin, SF;Hsu, JTA;Yang, CS;Tsai, CH;Takada, K;Chen, MR;Chen, JY |
| Contributors: | National Institute of Cancer Research;Center for Nanomedicine Research;Institute of Biotechnology and Pharmaceutical Research |
| Abstract: | Epstein-Barr virus (EBV) lytic replication involves complex processes, including DNA synthesis, DNA cleavage and packaging, and virion egress. These processes require many different lytic gene products, but the mechanisms of their actions remain unclear, especially for DNA cleavage and packaging. According to sequence homology analysis, EBV BALF3, encoded by the third leftward open reading frame of the BamHI-A fragment in the viral genome, is a homologue of herpes simplex virus type 1 UL28. This gene product is believed to possess the properties of a terminase, such as nucleolytic activity on newly synthesized viral DNA and translocation of unit length viral genomes into procapsids. In order to characterize EBV BALF3, the protein was produced by and purified from recombinant baculoviruses and examined in an enzymatic reaction in vitro, which determined that EBV BALF3 acts as an endonuclease and its activity is modulated by Mg2+, Mn2+, and ATP. Moreover, in EBV-positive epithelial cells, BALF3 was expressed and transported from the cytoplasm into the nucleus following induction of the lytic cycle, and gene silencing of BALF3 caused a reduction of DNA packaging and virion release. Interestingly, suppression of BALF3 expression also decreased the efficiency of DNA synthesis. On the basis of these results, we suggest that EBV BALF3 is involved simultaneously in DNA synthesis and packaging and is required for the production of mature virions. |
| Date: | 2014-02 |
| Relation: | Journal of Virology. 2014 Feb;88(9):4962-4975. |
| Link to: | http://dx.doi.org/10.1128/jvi.00063-14 |
| JIF/Ranking 2023: | http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0022-538X&DestApp=IC2JCR |
| Cited Times(WOS): | https://www.webofscience.com/wos/woscc/full-record/WOS:000334353900031 |
| Cited Times(Scopus): | http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84897495549 |
| Appears in Collections: | [陳振陽] 期刊論文
[林素芳] 期刊論文
[楊重熙] 期刊論文
[徐祖安] 期刊論文
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| SCP84897495549.pdf | | 2416Kb | Adobe PDF | 608 | View/Open |
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