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http://ir.nhri.org.tw/handle/3990099045/8048
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| Title: | Second worldwide proficiency study on variable number of tandem repeats typing of Mycobacterium tuberculosis complex |
| Authors: | De Beer, JL;Ködm̈on, C;Van Ingen, J;Supply, P;Van Soolingen, D;Jamieson, FB;Bidovec-Stojkovic, U;Brown, T;Cirillo, DM;Cruz, L;Miranda, A;Dou, HY;Fauville-Dufaux, M;Fitzgibbon, MM;García De Viedma, D;Groenheit, R;Haanperä-Heikkinen, M;Indra, A;Kam, KM;Kramer, R;Jiang, GL;Niemann, S;Obrovac, M;Rasmussen, EM;Refrégier, G;Realpe, T;Samper, S;Sharma, MK;Sougakoff, W;Stakenas, P;Stavrum, R;Trenkler, J;Wada, T;Siame, KK;Tafaj, S;Cowan, L;Sng, LH;Seagar, AL;Basu, I;Rastogi, N;Ferro, BE;De Matos, F;Kipnis, A |
| Contributors: | Division of Infectious Diseases |
| Abstract: | BACKGROUND: The quality of variable number of tandem repeats (VNTR) typing of Mycobacterium tuberculosis was first investigated in 2009 in 37 laboratories worldwide. The results revealed an inter- and intra-laboratory reproducibility of respectively 60% and 72%. These data spurred an improvement in laboratory-specific assays and global standardisation of VNTR typing. OBJECTIVE: To measure the effects of the technical improvements and increased standardisation, a test panel consisting of 30 M. tuberculosis complex DNA samples was distributed for VNTR typing in 41 participating laboratories from 36 countries. RESULTS: The inter- and intra-laboratory reproducibility increased overall to respectively 78% and 88%. The 33 laboratories that participated in both the first and second proficiency studies improved their inter- and intra-laboratory reproducibility from 62% and 72% to respectively 79% and 88%. The largest improvement in reproducibility was detected in 10 laboratories that use an in-house polymerase chain reaction technique and perform amplicon sizing using gel electrophoresis. Detailed error analysis revealed a reduction in the number of systematic errors, sample exchange events and non-amplifiable loci. CONCLUS ION: This second worldwide proficiency study indicates a substantial increase in the reproducibility of VNTR typing of M. tuberculosis. This will contribute to a more meaningful interpretation of molecular epidemiological and phylogenetic studies on the M. tuberculosis complex. |
| Date: | 2014-05-01 |
| Relation: | International Journal of Tuberculosis and Lung Disease. 2014 May 1;18(5):594-600. |
| Link to: | http://dx.doi.org/10.5588/ijtld.13.0531 |
| JIF/Ranking 2023: | http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=1027-3719&DestApp=IC2JCR |
| Cited Times(WOS): | https://www.webofscience.com/wos/woscc/full-record/WOS:000335627800018 |
| Cited Times(Scopus): | http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84896486105 |
| Appears in Collections: | [杜鴻運] 期刊論文
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| SCP84896486105.pdf | | 601Kb | Adobe PDF | 692 | View/Open |
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