國家衛生研究院 NHRI:Item 3990099045/8243
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    Please use this identifier to cite or link to this item: http://ir.nhri.org.tw/handle/3990099045/8243


    Title: Human FGF1 promoter is active in ependymal cells and dopaminergic neurons in the brains of F1B-GFP transgenic mice
    Authors: Chen, MS;Lin, HK;Chiu, H;Lee, DC;Chung, YF;Chiu, IM
    Contributors: Institute of Cellular and Systems Medicine
    Abstract: FGF1 is involved in multiple biological functions and exhibits the importance in neuroprotective effects. Our previous studies indicated that, in human brain and retina, the FGF1B promoter controlled the expression of FGF1. However, the exact function and regulation of FGF1 in brain is still unclear. Here, we generated F1B-GFP transgenic mice that expressed the GFP reporter gene under the control of human FGF1B promoter (-540 to +31). Using the fresh brain sections of F1B-GFP transgenic mice, we found that the F1B-GFP cells expressed strong fluorescent signals in the ventricular system throughout the brain. The results of immunohistochemistry further showed that two distinct populations of F1B-GFP+ cells existed in the brains of F1B-GFP transgenic mice. We demonstrated that one population of F1B-GFP+ cells was ependymal cells, which distributed along the entire ventricles, and the second population of F1B-GFP+ cells was neuronal cells that projected their long processes into multiple directions in specific areas of the brain. The double labeling of F1B-GFP+ cells and tyrosine hydroxylase indicated that a subpopulation of F1B-GFP+-neuronal cells was dopaminergic neurons. Importantly, these F1B-GFP+/TH+ cells were distributed in the main dopaminergic neuronal groups including hypothalamus, ventral tegmental area, and raphe nuclei. These results suggested that human FGF1B promoter was active in ependymal cells, neurons, and a portion of dopaminergic neurons. Thus, the F1B-GFP transgenic mice provide an animal model not only for studying FGF1 gene expression in vivo but also for understanding the role of FGF1 contribution in neurodegenerative disorders such as Parkinson's disease and Alzheimer's disease.
    Date: 2015-03
    Relation: Developmental Neurobiology. 2015 Mar;75(3):232-248.
    Link to: http://dx.doi.org/10.1002/dneu.22225
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=1932-8451&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000349612800002
    Cited Times(Scopus): http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84922763762
    Appears in Collections:[Ing-Ming Chiu] Periodical Articles

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