國家衛生研究院 NHRI:Item 3990099045/8253

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Title:	Closely related NDM-1-encoding plasmids from Escherichia coli and Klebsiella pneumoniae in Taiwan
Authors:	Chen, CJ;Wu, TL;Lu, PL;Chen, YT;Fung, CP;Chuang, YC;Lin, JC;Siu, LK
Contributors:	Division of Infectious Diseases;Institute of Molecular and Genomic Medicine
Abstract:	OBJECTIVE: Two plasmids carrying blaNDM-1 isolated from carbapenem-resistant Klebsiella pneumoniae (CR-KP) and carbapenem-resistant Escherichia coli (CR-EC) were sequenced. CR-KP and CR-EC were isolated from two Taiwanese patients without travel histories. METHODS: Complete sequencing of the plasmids (pLK75 and pLK78) was conducted using a shotgun approach. Annotation of the contigs was performed using the RAST Server, followed by manual inspection and correction. RESULTS: These similar plasmids were obtained from two patients with overlapping stays at the same hospital. The pLK75 and pLK78 plasmids were 56,489-bp and 56,072-bp in length, respectively. Plasmid annotation revealed a common backbone similar to the IncN plasmid pR46. The regions flanking the blaNDM-1 genes in these plasmids were very similar to plasmid pNDM-HU01 in Japan, which contains a complex class 1 integron located next to an ISCR1 element. The ISCR1 element has been suggested to provide a powerful mechanism for mobilising antibiotic resistance genes. CONCLUSION: Two indigenous NDM-1-producing Enterobacteriaceae cases were identified for the first time in Taiwan, highlighting the alarming introduction of NDM-1-producing Enterobacteriaceae in this region.
Date:	2014-08-21
Relation:	PLoS ONE. 2014 Aug 21;9(8):Article number e104899.
Link to:	http://dx.doi.org/10.1371/journal.pone.0104899
JIF/Ranking 2023:	http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=1932-6203&DestApp=IC2JCR
Cited Times(WOS):	https://www.webofscience.com/wos/woscc/full-record/WOS:000341106100028
Cited Times(Scopus):	http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84927634944
Appears in Collections:	[Leung-Kei Siu] Periodical Articles [Others] Periodical Articles

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