國家衛生研究院 NHRI:Item 3990099045/8359
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    Please use this identifier to cite or link to this item: http://ir.nhri.org.tw/handle/3990099045/8359


    Title: Downregulated miR329 and miR410 promote the proliferation and invasion of oral squamous cell carcinoma by targeting Wnt-7b
    Authors: Shiah, SG;Hsiao, JR;Chang, WM;Chen, YW;Jin, YT;Wong, TY;Huang, JS;Tsai, ST;Hsu, YM;Chou, ST;Yen, YC;Jiang, SS;Shieh, YS;Chang, IS;Hsiao, M;Chang, JY
    Contributors: National Institute of Cancer Research
    Abstract: MicroRNA (miRNA) dysregulation contributes widely to human cancer but has not been fully assessed in oral cancers. In this study, we conducted a global microarray analysis of miRNA expression in 40 pairs of betel quid-associated oral squamous cell carcinoma (OSCC) specimens and their matched non-tumorous epithelial counterparts. Eighty-four miRNAs were differentially expressed in the OSCC specimens compared to the matched tissue. Among these downregulated miRNAs, 19 miRNAs were found and mapped to the chromosome 14q32.2 miRNA cluster region, which resides within a parentally imprinted region designated Dlk-Dio3 and known to be important in development and growth. Bioinformatic analysis predicted two miRNAs from the cluster region, miR-329 and miR-410, which could potentially target Wnt-7b, an activator of the Wnt/beta-catenin pathway, thereby attenuating the Wnt/beta-catenin signaling pathway in OSCC. Stable ectopic expression of Wnt-7b in OSCC cells overexpressing miR-329 or miR-410 restored proliferation and invasion capabilities abolished by these miRNA. Combining a demethylation agent and a histone deacetylase inhibitor was sufficient to re-express miR-329, miR-410 and Meg3 consistent with epigenetic regulation of these miRNA in human OSCC. Specifically, arecoline, a major betel nut alkaloid, reduced miR-329, miR-410 and Meg3 gene expression. Overall, our results provide novel molecular insights into how betel quid contribute to oral carcinogenesis through epigenetic silencing of tumor suppressor miRNA which target Wnt/beta-catenin signaling.
    Date: 2014-12-15
    Relation: Cancer Research. 2014 Dec 15;74(24):7560-7572.
    Link to: http://dx.doi.org/10.1158/0008-5472.can-14-0978
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0008-5472&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000346363900038
    Cited Times(Scopus): http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84918491424
    Appears in Collections:[Jang-Yang Chang] Periodical Articles
    [I-Shou Chang] Periodical Articles
    [Shih-Sheng Jiang] Periodical Articles
    [Ya-Wen Chen] Periodical Articles
    [Shine-Gwo Shiah] Periodical Articles

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