國家衛生研究院 NHRI:Item 3990099045/8619
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    题名: Extended-spectrum cephalosporin resistant Escherichia coli in retail meat in Taiwan
    作者: Yang, LTL;Shiau, YR;Tan, MC;Lai, JF;Wang, HY;Huang, IW
    贡献者: Division of Infectious Diseases
    摘要: Purpose: Our national surveillance program indicated an increasing trend of extended-spectrum cephalosporin (ESC) resistance in E. coli from outpatients. Since antimicrobial resistant bacteria in the food supply may be transferred to humans through direct or indirect contact, we investigated the prevalence of ESC-resistant E. coli in retail meat in Taiwan. Methods: Meat samples were purchased in 2012 and 2013 from traditional markets in different regions of Taiwan. One gram of the meat was incubated in 10 ml buffered peptone water overnight and 10 μl of the broth was subcultured to MacConkey and ESBL selective agar plates. After species confirmation, antimicrobial susceptibility was determined using broth microdilution method. Extended-spectrum β-lactamase (ESBL) confirmatory test was performed and the presence of ESBL and AmpC β-lactamase genes (blaESBL & blaAmpC) was determined by PCR. Results: A total of 95 (48 in 2012 and 47 in 2013) meat samples were studied. ESC-resistant E. coli isolates were detected in 60 samples (63.2%), including 30.4%, 80%, and 64.9% of the 23, 35, and 37 beef, chicken and pork samples, respectively. A total of 85 non-duplicate ESC-resistant isolates were recovered. blaESBL was detected in 68 isolates from 50 (52.6%) meat samples, all carried CTX-M type (groups 1 and 9), and 8 samples contained both CTX-M group 1 and group 9 isolates. blaAmpC was detected in 18 isolates from 18 (18.9%) samples, all were CMY-type. Four isolates carried both blaCTX-M and blaCMY. Conclusions: CTX-M ESBL-producing E. coli is highly prevalent in retail meat, especially in chicken and pork, the most often consumed meat types in Taiwan. CMY-type AmpC–producing E. coli are also present in meat supplies. Since CTX-M and CMY are the most frequently identified β-lactamases in human clinical ESC-resistant E. coli isolates in Taiwan, further studies to determine the relatedness of the clinical and meat ESC-resistant isolates are warranted.
    日期: 2015-04
    關聯: Journal of Microbiology, Immunology and Infection. 2015 Apr;48(2, Suppl.1):S112.
    Link to: http://dx.doi.org/10.1016/j.jmii.2015.02.395
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=1684-1182&DestApp=IC2JCR
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