國家衛生研究院 NHRI:Item 3990099045/8627

NHRI > National Institute of Infectious Diseases and Vaccinology > Min-Shi Lee > Periodical Articles > Item 3990099045/8627

Please use this identifier to cite or link to this item: http://ir.nhri.org.tw/handle/3990099045/8627

Title:	Evaluation of MDCK cell-derived influenza H7N9 vaccine candidates in ferrets
Authors:	Chia, MY;Hu, AY;Tseng, YF;Weng, TC;Lai, CC;Lin, JY;Chen, PL;Wang, YF;Chao, SR;Chang, JY;Hwang, YS;Yeh, CT;Yu, CP;Chen, YC;Su, IJ;Lee, MS
Contributors:	Division of Infectious Diseases;Division of Vaccine Research and Development
Abstract:	Avian-origin influenza A (H7N9) viruses emerged as human pathogens in China in early 2013 and have killed >100 persons. Influenza vaccines are mainly manufactured using egg-based technology which could not meet the surging demand during influenza pandemics. In this study, we evaluated cell-based influenza H7N9 vaccines in ferrets. An egg-derived influenza H7N9 reassortant vaccine virus was adapted in MDCK cells. Influenza H7N9 whole virus vaccine antigen was manufactured using a microcarrier-based culture system. Immunogenicity and protection of the vaccine candidates with three different formulations (300mug aluminum hydroxide, 1.5mug HA, and 1.5mug HA plus 300mug aluminum hydroxide) were evaluated in ferrets. In ferrets receiving two doses of vaccination, geometric mean titers of hemagglutination (HA) inhibition and neutralizing antibodies were <10 and <40 for the control group (adjuvant only), 17 and 80 for the unadjuvanted (HA only) group, and 190 and 640 for the adjuvanted group (HA plus adjuvant), respectively. After challenge with wild-type influenza H7N9 viruses, virus titers in respiratory tracts of the adjuvanted group were significantly lower than that in the control, and unadjuvanted groups. MDCK cell-derived influenza H7N9 whole virus vaccine candidate is immunogenic and protective in ferrets and clinical development is highly warranted.
Date:	2015-03-23
Relation:	PLoS ONE. 2015 Mar 23;10(3):Article number e0120793.
Link to:	http://dx.doi.org/10.1371/journal.pone.0120793
JIF/Ranking 2023:	http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=1932-6203&DestApp=IC2JCR
Cited Times(WOS):	https://www.webofscience.com/wos/woscc/full-record/WOS:000351987300178
Cited Times(Scopus):	http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84925813058
Appears in Collections:	[Min-Shi Lee] Periodical Articles [Ih-Jen Su(2002-2015)] Periodical Articles [Yee-Chun Chen] Periodical Articles [Yung-Chih Hu] Periodical Articles

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