國家衛生研究院 NHRI:Item 3990099045/8751
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    题名: Synergistic effect of PEI and PDMAEMA on transgene expression in vitro
    作者: Lo, CW;Liao, WH;Wu, CH;Lee, JL;Sun, MK;Yang, HS;Tsai, WB;Chang, Y;Chen, WS
    贡献者: Institute of Biomedical Engineering and Nanomedicine
    摘要: Polyethylenimine (PEI) and poly(2-(dimethylamino) ethyl methacrylate) (PDMAEMA) have both been used for DNA delivery. PDMAEMA has been shown to exhibit better gene transfection efficiency but lower expression ability than PEI. We mixed the two polymers at different ratios to investigate whether the resulting "dual" polyplex (PEI/PDMAEMA/DNA) could enhance both gene transfection efficiency and DNA expression ability. Experimental results showed a significant increase in DNA internalization and DNA expression for the PDMAEMA/PEI/DNA polyplexes at a ratio of 1:3 or 1:9 (PDMAEMA: PEI), depending on cell type, in comparison with PEI/DNA, PDMAEMA/DNA, and PDMAEMA/PEI/DNA at other ratios. PDMAEMA/PEI/DNA polyplexes did not reduce cell viability. In contrast to with the conventional approach using covalently modified PEI, the proposed "combination" approach provided a more convenient and effective way to improve transgene expression efficiency.
    日期: 2015-06
    關聯: Langmuir. 2015 Jun;31(22):6130-6136.
    Link to: http://dx.doi.org/10.1021/acs.langmuir.5b00520
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0743-7463&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000356202300020
    Cited Times(Scopus): http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84931281695
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