國家衛生研究院 NHRI:Item 3990099045/8757
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    題名: A microfluidic dual-well device for high-throughput single-cell capture and culture
    作者: Lin, CH;Hsiao, YH;Chang, HC;Yeh, CF;He, CK;Salm, EM;Chen, CC;Chiu, IM;Hsu, CH
    貢獻者: Institute of Biomedical Engineering and Nanomedicine;Institute of Cellular and Systems Medicine
    摘要: In vitro culture of single cells facilitates biological studies by deconvoluting complications from cell population heterogeneity. However, there is still a lack of simple yet high-throughput method to perform single cell culture experiment. In this paper, we report the development and application of a microfluidic device with a dual-well (DW) design concept for high-yield single-cell loading (~77%) in large microwells (285 and 485 [small mu ]m in diameter) which allowed for cell spreading, proliferation and differentiation. The increased single-cell-loading yield is achieved by using sets of small microwells termed "capture-wells" and big microwells termed "culture-wells" according to their utilities for single-cell capture and culture respectively. This novel device architecture allows the size of the "culture" microwells to be flexibly adjusted without affecting the single-cell loading efficiency making it useful for cell culture applications as demonstrated by our experiments of KT98 mouse neural stem cell differentiation, A549 and MDA-MB-435 cancer cell proliferation, and a single-cell colony formation assay with A549 cells in this paper.
    日期: 2015-07
    關聯: Lab on a Chip. 2015 Jul;15(14):2928-2938.
    Link to: http://dx.doi.org/10.1039/C5LC00541H
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=1473-0197&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000357177300003
    Cited Times(Scopus): http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84934282098
    顯示於類別:[許佳賢] 期刊論文
    [邱英明] 期刊論文

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