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    Please use this identifier to cite or link to this item: http://ir.nhri.org.tw/handle/3990099045/8995


    Title: 重組桿狀病毒感染非宿主細胞的方法
    Other Titles: Method of infecting non-permissive cells with a recombinant baculovirus
    Authors: Juang, JL;Lee, DF
    Contributors: Institute of Molecular and Genomic Medicine
    Abstract: 一種以重組病毒為主之載體,如重組桿狀病毒的組成和建構方法,使非宿主細胞表現外源目標蛋白質,而不表現可偵測之選擇標記子。重組載體包括一第一核酸序列編譯可偵測之選擇標記子受第一啟動子所操控,其中,第一啟動子在用於篩選細胞之宿主細胞內具有活性,但在表現外源目標蛋白質之非宿主細胞內則沒有活性。載體還包括一外源核酸序列在非宿主細胞內具有活性之啟動子的控制之下編譯目標蛋白質。本發明之系統可使選擇標記子在病毒斑篩選時表現,但在產出目標蛋白質時則不表現。
    Date: 2010-09-11
    Relation: I330198/2010-09-11
    Link to: https://twpat1.tipo.gov.tw/tipotwoc/tipotwkm?!!FR_I330198
    Appears in Collections:[莊志立] 專利

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