國家衛生研究院 NHRI:Item 3990099045/9126
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    題名: Effective transfer of a 47 kb NDM-1-positive plasmid among Acinetobacter species
    作者: Huang, TW;Lauderdale, TL;Liao, TL;Hsu, MC;Chang, FY;Chang, SC;Khong, WX;Ng, OT;Chen, YT;Kuo, SC;Chen, TL;Mu, JJ;Tsai, SF
    貢獻者: Institute of Molecular and Genomic Medicine;Division of Infectious Diseases
    摘要: OBJECTIVES: To investigate the link between two NDM-1-positive Acinetobacter isolates from the same hospital, the plasmid profiles of the isolates were examined. These two isolates were found from a surveillance programme within 3 months from two patients without obvious physical contact or hospitalization time overlap. METHODS: Antimicrobial susceptibility tests, genome sequencing of both isolates and plasmid transfer experiments were performed. A comparative study of similar plasmids was performed using BLAST analysis. RESULTS: The antimicrobial susceptibility of the isolates (Acinetobacter soli M131 and Acinetobacter pittii MS32) and their Escherichia coli transconjugants revealed a conjugative plasmid that carried the carbapenem resistance determinant. Eleven plasmids were observed in M131 and three in MS32. Each isolate shared an identical plasmid that carried the blaNDM-1 gene. This 47 271 bp plasmid harbours a conserved blaNDM-1-containing region that is flanked by ISAba125 and ISAba11 elements, and also contains a Ti-type conjugative operon. The plasmid is nearly identical in sequence to those of Acinetobacter isolates from China. In contrast to the mobilization of the blaNDM-1 sequence in Enterobacteriaceae, which is mainly by transposition, this plasmid moves as a whole among Acinetobacter species. Consistently, this plasmid was found to transfer effectively by in vitro conjugation to several Acinetobacter species. CONCLUSIONS: The clinical and laboratory findings suggest that Acinetobacter species may serve as a reservoir of this blaNDM-1 plasmid. Our study demonstrates the potential of applying genome sequencing to the surveillance of antimicrobial-resistant bacteria.
    日期: 2015-10
    關聯: Journal of Antimicrobial Chemotherapy. 2015 Oct;70(10):2734-2738.
    Link to: http://dx.doi.org/10.1093/jac/dkv191
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0305-7453&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000363249800008
    Cited Times(Scopus): http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84943782009
    顯示於類別:[蔡世峯] 期刊論文
    [郭書辰] 期刊論文
    [楊采菱] 期刊論文

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