國家衛生研究院 NHRI:Item 3990099045/9325

NHRI > National Institute of Infectious Diseases and Vaccinology > Ming-Hsi Huang > Periodical Articles > Item 3990099045/9325

Please use this identifier to cite or link to this item: http://ir.nhri.org.tw/handle/3990099045/9325

Title:	Design and development of immunomodulatory antigen delivery systems based on peptide/PEG-PLA conjugate for tuning immunity
Authors:	Coumes, F;Huang, CY;Huang, CH;Coudane, J;Domurado, D;Li, S;Darcos, V;Huang, MH
Contributors:	Division of Vaccine Research and Development
Abstract:	Cancer vaccines are considered to be a promising tool for cancer immunotherapy. However, a well-designed cancer vaccine should combine a tumor-associated antigen (TAA) with the most effective immunomodulatory agents and/or delivery system to provoke intense immune responses against the TAA. In the present study, we introduced a new approach by conjugating the immunomodulatory molecule LD-indolicidin to the hydrophilic chain end of the polymeric emulsifier poly(ethylene glycol)-polylactide (PEG-PLA), allowing the molecule to be located close to the surface of the resulting emulsion. A peptide/polymer conjugate, named LD-indolicidin-PEG-PLA, was synthesized by conjugation of the amine end-group of LD-indolicidin to the N-hydroxysuccinimide-activated carboxyl end-group of PEG. As an adjuvant for cancer immunotherapeutic use, TAA vaccine candidate formulated with the LD-indolicidin-PEG-PLA-stabilized squalene-in-water emulsion could effectively help to elicit a T helper (Th)1-dominant antigen-specific immune response as well as antitumor ability, using ovalbumin (OVA) protein/EG7 cells as a TAA/tumor cell model. Taken together, these results open up a new approach to the development of immunomodulatory antigen delivery systems for vaccine adjuvants and cancer immunotherapy technologies.
Date:	2015-11
Relation:	Biomacromolecules. 2015 Nov;16(11):3666-3673.
Link to:	http://dx.doi.org/10.1021/acs.biomac.5b01150
JIF/Ranking 2023:	http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=1525-7797&DestApp=IC2JCR
Cited Times(WOS):	https://www.webofscience.com/wos/woscc/full-record/WOS:000364614300027
Cited Times(Scopus):	http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84946811813
Appears in Collections:	[Ming-Hsi Huang] Periodical Articles

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