國家衛生研究院 NHRI:Item 3990099045/9506
English  |  正體中文  |  简体中文  |  全文筆數/總筆數 : 12189/12972 (94%)
造訪人次 : 966838      線上人數 : 697
RC Version 6.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
搜尋範圍 查詢小技巧：
  • 您可在西文檢索詞彙前後加上"雙引號"，以獲取較精準的檢索結果
  • 若欲以作者姓名搜尋，建議至進階搜尋限定作者欄位，可獲得較完整資料
    •  進階搜尋
    主頁 ‧ 登入 ‧ 上傳 ‧ 說明 ‧ 關於NHRI ‧ 管理 到手機版


    請使用永久網址來引用或連結此文件: http://ir.nhri.org.tw/handle/3990099045/9506


    題名: Guidelines for the use and interpretation of assays for monitoring autophagy
    作者: Klionsky, DJ;Abdelmohsen, K;Abe, A;Abedin, MJ;Abeliovich, H;Acevedo Arozena, A;Adachi, H;Adams, CM;Adams, PD;Adeli, K;Adhihetty, PJ;Adler, SG;Agam, G;Agarwal, R;Aghi, MK;Agnello, M;Agostinis, P;Aguilar, PV;Aguirre-Ghiso, J;Airoldi, EM;Ait-Si-Ali, S;Akematsu, T;Akporiaye, ET;Al-Rubeai, M;Albaiceta, GM;Albanese, C;Albani, D;Albert, ML;Aldudo, J;Algul, H;Alirezaei, M;Alloza, I;Almasan, A;Almonte-Beceril, M;Alnemri, ES;Alonso, C;Altan-Bonnet, N;Altieri, DC;Alvarez, S;et al.
    貢獻者: Institute of Molecular and Genomic Medicine;Immunology Research Center;Institute of Biotechnology and Pharmaceutical Research
    摘要: In 2008 we published the first set of guidelines for standardizing research in autophagy. Since then, research on this topic has continued to accelerate, and many new scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Accordingly, it is important to update these guidelines for monitoring autophagy in different organisms. Various reviews have described the range of assays that have been used for this purpose. Nevertheless, there continues to be confusion regarding acceptable methods to measure autophagy, especially in multicellular eukaryotes. A key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers or volume of autophagic elements (e.g., autophagosomes or autolysosomes) at any stage of the autophagic process vs. those that measure flux through the autophagy pathway (i.e., the complete process); thus, a block in macroautophagy that results in autophagosome accumulation needs to be differentiated from stimuli that result in increased autophagic activity, defined as increased autophagy induction coupled with increased delivery to, and degradation within, lysosomes (in most higher eukaryotes and some protists such as Dictyostelium) or the vacuole (in plants and fungi). In other words, it is especially important that investigators new to the field understand that the appearance of more autophagosomes does not necessarily equate with more autophagy. In fact, in many cases, autophagosomes accumulate because of a block in trafficking to lysosomes without a concomitant change in autophagosome biogenesis, whereas an increase in autolysosomes may reflect a reduction in degradative activity. Here, we present a set of guidelines for the selection and interpretation of methods for use by investigators who aim to examine macroautophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes. These guidelines are not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to monitor autophagy. In these guidelines, we consider these various methods of assessing autophagy and what information can, or cannot, be obtained from them. Finally, by discussing the merits and limits of particular autophagy assays, we hope to encourage technical innovation in the field.
    日期: 2016-01-02
    關聯: Autophagy. 2016 Jan 2;12(1):1-222.
    Link to: http://dx.doi.org/10.1080/15548627.2015.1100356
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=1554-8627&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000373595400001
    Cited Times(Scopus): https://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=85013763791
    顯示於類別:[龔行健] 期刊論文
    [莊宗顯] 期刊論文
    [李秀珠] 期刊論文

    文件中的檔案:

    檔案 描述 大小格式瀏覽次數
    PUB26799652.pdf7681KbAdobe PDF743檢視/開啟


    在NHRI中所有的資料項目都受到原著作權保護.

    TAIR相關文章

    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - 回饋