國家衛生研究院 NHRI:Item 3990099045/9508

NHRI > National Institute of Infectious Diseases and Vaccinology > Hsin-Wei Chen > Periodical Articles > Item 3990099045/9508

Please use this identifier to cite or link to this item: http://ir.nhri.org.tw/handle/3990099045/9508

Title:	Recombinant lipidated dengue-3 envelope protein domain III stimulates broad immune responses in mice
Authors:	Chiang, CY;Liu, SJ;Hsieh, CH;Chen, MY;Tsai, JP;Liu, HH;Chen, IH;Chong, P;Leng, CH;Chen, HW
Contributors:	Division of Vaccine Research and Development
Abstract:	The linkage of an immunogen with a toll-like receptor ligand has great potential to induce highly potent immune responses with the initial features of antigen-presenting cell activation. In the current study, we expressed recombinant dengue-3 envelope protein domain III (D3ED III) in lipidated form using an Escherichia coli-based system. The recombinant lipidated dengue-3 envelope protein domain III (LD3ED III) augments the expression levels of IL-12 family cytokines. LD3ED III-immunized mice enhance wide ranges of T cell responses as indicated by IFN-γ, IL-17, IL-21 production. Additionally, LD3ED III-immunized mice increase the frequencies of anti-D3ED III antibody producing cells. The boosted antibody titers cover various IgG isotypes, including IgG1, IgG2a, IgG2b, and IgG3. Importantly, LD3ED III-immunized mice induce neutralizing antibody capacity associated with a reduction of viremia levels after challenges. In contrast, mice that are immunized with D3ED III formulated with aluminum phosphate (D3ED III/Alum) only enhance Th2 responses and boost IgG1 antibody titers. Neither neutralizing antibody responses nor the inhibition of viremia levels after challenge is observed in mice that are immunized with D3ED III/Alum. These results suggest that LD3ED III can induce broad profiles of cellular and humoral immune responses.
Date:	2016-02
Relation:	Vaccine. 2016 Feb;34(8):1054-1061.
Link to:	http://dx.doi.org/10.1016/j.vaccine.2016.01.009
JIF/Ranking 2023:	http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0264-410X&DestApp=IC2JCR
Cited Times(WOS):	https://www.webofscience.com/wos/woscc/full-record/WOS:000371101100010
Cited Times(Scopus):	http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84955280136
Appears in Collections:	[Hsin-Wei Chen] Periodical Articles [Chih-Hsiang Leng] Periodical Articles [Pele Choi-Sing Chong] Periodical Articles [Shih-Jen Liu] Periodical Articles

Files in This Item:

File	Description	Size	Format
SDO0264410X16000281.pdf		1456Kb	Adobe PDF	545	View/Open

Loading...