Background and Aim: This study aimed to implement sequence driven analyses of IgG repertoires to comprehend chronic hepatitis B infections and immune tolerance to vaccination. Methods: Repertoires were prepared from four carrier – noncarrier sibling pairs both before and 2 weeks after vaccination as sequence sets of the complementarity-determining region 3 (CDR3) on IgG heavy chains. Analyses were carried out in a framework shaped by both CDR3 clusters and diversity measures modeled from numerical ecology. Results: Carriers ’ repertoires clustered in principal component analysis (Fig. A). A huge set of related CDR3 clones presented almost exclusively among carriers (Fig. B). Vaccination increased CDR3 diversities among noncarriers (Fig. C), and tolerance at focused spectra of carriers ’ repertoires was distinguishable with post-vaccination clusters from noncarriers (Fig. D). Conclusion: Both vaccine tolerance and virus intolerance in children with chronic hepatitis B infections were distinct features strongly perceivable at perspectives of diversity measures and sequence clusters, demonstrating great potentials of immune repertoires in disease annotations.
Date:
2015-12
Relation:
Journal of Gastroenterology and Hepatology. 2015 Dec;30(Suppl. S4):23.
