國家衛生研究院 NHRI:Item 3990099045/9655
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    题名: Enzyme-free dissociation of neurospheres by a microfluidic chip-based method
    作者: Lin, CH;Chang, HC;Hsu, CH
    贡献者: Institute of Biomedical Engineering and Nanomedicine;Institute of Cellular and Systems Medicine
    摘要: Neurosphere assay is a common and robust method for identification of neural stem/progenitor cells, but obtaining large numbers of live single cells from dissociated neurospheres is difficult using nonenzymatic methods. Here, we present an enzyme-free method for high-efficiency neurosphere dissociation into single cells using microfluidic device technology. This method allows single cell dissociation of DC115 and KT98 cells with high cell viabilities (80-85 %), single-cell yield (91-95 %), and recovery (75-93 %).
    日期: 2016-04-05
    關聯: Methods in Molecular Biology. 2016 Apr 5;1516:289-297.
    Link to: http://dx.doi.org/10.1007/7651_2016_348
    显示于类别:[許佳賢] 期刊論文
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