國家衛生研究院 NHRI:Item 3990099045/9764
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    题名: Cellular and functional heterogeneity of the sarcomatoid renal cell carcinoma cell line RCC52 with total loss of HLA class I: A system to study mesenchymalepithelial transition
    作者: Hsieh, CH;Chuang, KL;Yeh, CT;Chuang, CK;Pang, ST;Liao, SK
    贡献者: National Institute of Cancer Research
    摘要: RCC52, a cell line was established from a primary lesion of a sarcomatoid renal cell carcinoma (RCC). We have previously reported that this total loss of HLA class I was caused by the coexistence of the two mutations on the \#946;-2 microglobulin (\#946;2m) genes. These two mutations were located in different alleles in grossly fibroblast-like and epithelioid clonal subsets, as summerized in Table below. To analysis of these sublines further, our loss of heterozygosity (LOH) results indicate that the epithelioid cells have more extensive genomic instability than the fibroblast-like cells, since the former carry one small deletion at \#946;2m and one large mutation (LOH) at chromosome 15, whereas the latter have two separate small mutations at \#946;2m with heterozygosity still maintained (ROH). The more advanced stage of epithelioid cells was supported by their faster growth rate in vitro and readily growth in SCID mice, as well as their predominance in the tumor lesion and in the cultured cells, compared with fibroblast-like cells. However, it is not clear whether the two morphologically distinct types of cells are two unrelated tumor subsets evolved together at the tumor site, or were in the process of a mesenchymal-epithelial transition (MET) when the disease progressed. Thus far, MET is rare compared with EMT, which has been an indication for the progression of carcinoma towards dedifferentiation and a more malignant status in other tumor types. RT-PCR analysis of the expression of Hh-associated pathway components revealed that with the exception of Hip, PTCH, SMO, Ihh, and Gli1 generally exhibited higher in epithelioid sublines. Experiments with use of this cell line are currently being conducted to determine which subset will display S100A4 with concomitant increased expression of the transcription factor Ets1. The RCC52 cell line may provide a useful model for studying the role of MET and/or EMT in tumor progression and metastasis.
    日期: 2009-05
    關聯: Cancer Research. 2009 May;69:Abstract number 222.
    Link to: http://cancerres.aacrjournals.org/content/69/9_Supplement/222.short
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0008-5472&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000209702603407
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