國家衛生研究院 NHRI:Item 3990099045/9872

NHRI > Institute of Biomedical Engineering and Nanomedicine > Chia-Hsien Hsu > Periodical Articles > Item 3990099045/9872

Please use this identifier to cite or link to this item: http://ir.nhri.org.tw/handle/3990099045/9872

Title:	A PDMS-based microfluidic hanging drop chip for embryoid body formation
Authors:	Wu, HW;Hsiao, YH;Chen, CC;Yet, SF;Hsu, CH
Contributors:	Institute of Biomedical Engineering and Nanomedicine;Institute of Cellular and Systems Medicine
Abstract:	The conventional hanging drop technique is the most widely used method for embryoid body (EB) formation. However, this method is labor intensive and limited by the difficulty in exchanging the medium. Here, we report a microfluidic chip-based approach for high-throughput formation of EBs. The device consists of microfluidic channels with 6 x 12 opening wells in PDMS supported by a glass substrate. The PDMS channels were fabricated by replicating polydimethyl-siloxane (PDMS) from SU-8 mold. The droplet formation in the chip was tested with different hydrostatic pressures to obtain optimal operation pressures for the wells with 1000 mum diameter openings. The droplets formed at the opening wells were used to culture mouse embryonic stem cells which could subsequently developed into EBs in the hanging droplets. This device also allows for medium exchange of the hanging droplets making it possible to perform immunochemistry staining and characterize EBs on chip.
Date:	2016-07-06
Relation:	Molecules. 2016 Jul 6;21(7):Article number 882.
Link to:	http://dx.doi.org/10.3390/molecules21070882
JIF/Ranking 2023:	http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=1420-3049&DestApp=IC2JCR
Cited Times(WOS):	https://www.webofscience.com/wos/woscc/full-record/WOS:000381509700067
Cited Times(Scopus):	http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84979036042
Appears in Collections:	[Chia-Hsien Hsu] Periodical Articles [Shaw-Fang Yet] Periodical Articles

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