國家衛生研究院 NHRI:Item 3990099045/9921
English  |  正體中文  |  简体中文  |  全文筆數/總筆數 : 12340/13424 (92%)
造訪人次 : 2036742      線上人數 : 473
RC Version 6.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
搜尋範圍 查詢小技巧：
  • 您可在西文檢索詞彙前後加上"雙引號"，以獲取較精準的檢索結果
  • 若欲以作者姓名搜尋，建議至進階搜尋限定作者欄位，可獲得較完整資料
    •  進階搜尋
    主頁 ‧ 登入 ‧ 上傳 ‧ 說明 ‧ 關於NHRI ‧ 管理 到手機版


    請使用永久網址來引用或連結此文件: http://ir.nhri.org.tw/handle/3990099045/9921


    題名: Cardiac fibrosis in mouse expressing DsRed tetramers involves chronic autophagy and proteasome degradation insufficiency
    作者: Chen, TH;Chen, MR;Chen, TY;Wu, TC;Liu, SW;Hsu, CH;Liou, GG;Kao, YY;Dong, GC;Chu, PH;Liao, JW;Lin, KM
    貢獻者: Institute of Biomedical Engineering and Nanomedicine;Institute of Molecular and Genomic Medicine
    摘要: Proteinopathy in the heart which often manifests excessive misfolded/aggregated proteins in cardiac myocytes can result in severe fibrosis and heart failure. Here we developed a mouse model, which transgenically express tetrameric DsRed, a red fluorescent protein (RFP), in an attempt to mimic the pathological mechanisms ofcardiac fibrosis. Whilst DsRed is expressed and forms aggregation in most mouse organs, certain pathological defects are specifically recapitulated in cardiac muscle cells including mitochondria damages, aggresome-like residual bodies, excessive ubiquitinated proteins, and the induction of autophagy. The proteinopathy and cellular injuries caused by DsRed aggregates may be due to impaired or overburdened ubiquitin-proteasome system and autophagy-lysosome systems. We further identified that DsRed can be ubiquitinated and associated with MuRF1, a muscle-specific E3 ligase. Concomitantly, an activation of NF-kappaB signaling and a strong TIMP1 induction were noted, suggesting that RFP-induced fibrosis was augmented by a skewed balance between TIMP1 and MMPs. Taken together, our study highlights the molecular consequences of uncontrolled protein aggregation leading to congestive heart failure, and provides novel insights into fibrosis formation that can be exploited for improved therapy.
    日期: 2016-08-02
    關聯: Oncotarget. 2016 Aug 2;7(34):54274-54289.
    Link to: http://dx.doi.org/10.18632/oncotarget.11026
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000385435000016
    Cited Times(Scopus): http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84983568259
    顯示於類別:[林名釗] 期刊論文
    [董國忠] 期刊論文
    [劉淦光(2006-2014)] 期刊論文

    文件中的檔案:

    檔案 描述 大小格式瀏覽次數
    PUB27494843.pdf9478KbAdobe PDF559檢視/開啟


    在NHRI中所有的資料項目都受到原著作權保護.

    TAIR相關文章

    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - 回饋