國家衛生研究院 NHRI:Item 3990099045/9974
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    題名: Morphology and gene expression screening with morpholinos in zebrafish embryos
    作者: Tseng, LC;Tang, CH;Jiang, YJ
    貢獻者: Institute of Molecular and Genomic Medicine
    摘要: High-throughput screening with a loss-of-function strategy is a logical and efficient way to identify novel genes involved in biological processes of interest. In zebrafish, morpholinos have been developed as a convenient tool to knock down gene expression. Here, we describe procedures for systematic screening using morpholinos in zebrafish to identify novel deubiquitylases involved in convergent extension during gastrulation. In this example, we examine candidates based on embryonic morphology and molecular signals of whole mount in situ hybridization assay.
    日期: 2016-09-02
    關聯: Methods in Molecular Biology. 2016 Sep 02;1470:213-224.
    Link to: http://dx.doi.org/10.1007/978-1-4939-6337-9_17
    顯示於類別:[江運金] 期刊論文

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