國家衛生研究院 NHRI:Item 3990099045/9991
English  |  正體中文  |  简体中文  |  全文筆數/總筆數 : 12145/12927 (94%)
造訪人次 : 924641      線上人數 : 909
RC Version 6.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
搜尋範圍 查詢小技巧：
  • 您可在西文檢索詞彙前後加上"雙引號"，以獲取較精準的檢索結果
  • 若欲以作者姓名搜尋，建議至進階搜尋限定作者欄位，可獲得較完整資料
    •  進階搜尋
    主頁 ‧ 登入 ‧ 上傳 ‧ 說明 ‧ 關於NHRI ‧ 管理 到手機版


    請使用永久網址來引用或連結此文件: http://ir.nhri.org.tw/handle/3990099045/9991


    題名: 5-Methoxytryptophan reduces neointima formation following arterial injury
    作者: Chen, CH;Ho, YC;Ho, HH;Su, CH;Yet, SF
    貢獻者: Institute of Cellular and Systems Medicine
    摘要: Objectives: In response to injury, vascular smooth muscle cells (VSMCs) undergo a phenotypic transition whereby they proliferate and migrate from the medial layer into the intima, contributing to neointima formation. 5-Methoxytryptophan (5-MTP) is a 5-methoxyindole metabolite of tryptophan metabolism, was recently shown to suppress inflammatory mediator-induced cancer cell proliferation and migration. However, the role of 5-MTP in vascular cells is unclear. The aim of this study was to investigate whether 5-MTP plays a role in modulating VSMC phenotypes and vascular remodeling following arterial injury. Methods: C57BL/6 mice were subjected to femoral artery endoluminal injury, treated with vehicle or 5-MTP, and arteries harvested 4 weeks later for histological analysis. Proinflammatory cytokine interleukin-1β (IL-1β) is increased in the injured vessel wall, thus we examined whether IL-1β affected VSMC phenotypic modulation. Primary cultured VSMCs were treated with or without IL-1β in the presence or absence of 5-MTP. The effects of 5-MTP on IL-1β-mediated VSMC proliferation, marker gene expressions, and signaling pathways were examined. Results: H&E and Verhoeff’s elastin staining revealed that compared with vehicle-treated mice, 5-MTP significantly decreased intimal thickening after arterial injury. Immunostaining of arterial sections showed that 5-MTP attenuated injury-mediated matrix metalloproteinase-2 (MMP2) upregulation and smooth muscle (SM) α-actin downregulation. Western blot analysis revealed that 5-MTP prevented IL-1β-mediated alterations of SM α-actin and MMP2. Furthermore, 5-MTP decreased IL-1β-induced cell proliferation and p38 MAPK activation. Conclusions: Our data indicate a protective role of 5-MTP against arterial injury-induced neointimal formation. Mechanistically, 5-MTP might exert its vascular effects via modulating VSMC phenotypes through p38 MAPK pathway.
    日期: 2016-09
    關聯: Atherosclerosis. 2016 Sep;252:e200.
    Link to: http://dx.doi.org/10.1016/j.atherosclerosis.2016.07.117
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0021-9150&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000388978400677
    顯示於類別:[林秀芳] 會議論文/會議摘要

    文件中的檔案:

    檔案 描述 大小格式瀏覽次數
    SDO002191501630418X.pdf47KbAdobe PDF406檢視/開啟


    在NHRI中所有的資料項目都受到原著作權保護.

    TAIR相關文章

    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - 回饋