Aims: Poly lactic-co-glycolic acid (PLGA) is a biocompatible and biodegradable material used in many biomedical applications, including bioactive agent delivery. In this study, we evaluated the immunogenicity of a particulate dengue subunit vaccine formulated by cationic PLGA microparticles adsorbed with recombinant dengue envelope protein domain III. Methods: Cationic PLGA microparticles were prepared by solvent evaporation in the water-phase containing a cationic surfactant-cetyltrimethyl ammonium bromide. The PLGA particulate dengue vaccine was formulated by incubation of cationic PLGA microparticles and recombinant dengue subunit protein. Results: The average size and zeta potential of PLGA particles were about 1 l m in diameter and + 22 mV. Mice were immunized by intramuscular injection three times with either PLGA formulated or soluble dengue vaccine. A significant increase of phagocytes and a pro-longed time for antigen release were detected in mice immunized with PLGA particulate dengue subunit vaccine. In addition, the specific IFN- c and IL-4 responses, as well as the anti-dengue IgG titers were significantly increased in mice immunized with PLGA particulate dengue subunit vaccine. Conclusions: In summary, the PLGA formulated particulate dengue subunit vaccine can enhance the immunogenicity of subunit vaccine by stimulating phagocytes to uptake antigen and depositing the antigen to extend the stimulation time.
Date:
2016-10
Relation:
Basic and Clinical Pharmacology and Toxicology. 2016 Oct;119(Suppl. 2):35.
