國家衛生研究院 NHRI:Item 3990099045/10089
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    题名: Development of dengue vaccine by using plga microparticle adsorbed with dengue subunit protein
    作者: Pan, CH;Hsieh, CH
    贡献者: Division of Vaccine Research and Development
    摘要: Aims: Poly lactic-co-glycolic acid (PLGA) is a biocompatible and biodegradable material used in many biomedical applications, including bioactive agent delivery. In this study, we evaluated the immunogenicity of a particulate dengue subunit vaccine formulated by cationic PLGA microparticles adsorbed with recombinant dengue envelope protein domain III. Methods: Cationic PLGA microparticles were prepared by solvent evaporation in the water-phase containing a cationic surfactant-cetyltrimethyl ammonium bromide. The PLGA particulate dengue vaccine was formulated by incubation of cationic PLGA microparticles and recombinant dengue subunit protein. Results: The average size and zeta potential of PLGA particles were about 1 l m in diameter and + 22 mV. Mice were immunized by intramuscular injection three times with either PLGA formulated or soluble dengue vaccine. A significant increase of phagocytes and a pro-longed time for antigen release were detected in mice immunized with PLGA particulate dengue subunit vaccine. In addition, the specific IFN- c and IL-4 responses, as well as the anti-dengue IgG titers were significantly increased in mice immunized with PLGA particulate dengue subunit vaccine. Conclusions: In summary, the PLGA formulated particulate dengue subunit vaccine can enhance the immunogenicity of subunit vaccine by stimulating phagocytes to uptake antigen and depositing the antigen to extend the stimulation time.
    日期: 2016-10
    關聯: Basic and Clinical Pharmacology and Toxicology. 2016 Oct;119(Suppl. 2):35.
    Link to: http://dx.doi.org/10.1111/bcpt.12659
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=1742-7835&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000385818700105
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